Merola P, Marino C, Lovisolo G A, Pinto R, Laconi C, Negroni A
Ente Nuove tecnologie Energia e Ambiente (ENEA), Section of Toxicology and Biomedical Sciences, Rome, Italy.
Bioelectromagnetics. 2006 Apr;27(3):164-71. doi: 10.1002/bem.20201.
The aim of this study was to examine whether a modulated radiofrequency of the type used in cellular phone communications at a specific absorption rate (SAR) higher than International Commission on Non-ionizing Radiation Protection (ICNIRP) reference level for occupational exposure, could elicit alterations on proliferation, differentiation, and apoptosis processes in a neuroblastoma cell line. The cell line was exposed for 24, 48, and 72 h to 900 MHz radiofrequency and proliferation and differentiation were tested by WST-I assay and by a molecular analysis of specific markers, two oncogenes and a cytoskeleton protein, in exponential growth phase and in synchronized cell cultures. Apoptosis was evaluated by caspase activation analysis and by molecular detection of Poly (ADP-ribose) polimerase (PARP) cleavage. Combined exposures to radiofrequency and to the differentiative agent retinoic acid or to the apoptotic inducer camptothecin were carried out to test possible interference between electromagnetic field and chemical agents. Overall our data suggest that 900 MHz radiofrequency exposure up to 72 h does not induce significant alterations in the three principal cell activities in a neuroblastoma cell line.
本研究的目的是检验在比国际非电离辐射防护委员会（ICNIRP）职业暴露参考水平更高的特定吸收率（SAR）下，手机通信中使用的那种调制射频，是否会引起神经母细胞瘤细胞系在增殖、分化和凋亡过程中的改变。该细胞系在指数生长期和同步化细胞培养物中，分别暴露于900 MHz射频24、48和72小时，然后通过WST-1检测以及对特定标志物、两个癌基因和一种细胞骨架蛋白进行分子分析来检测增殖和分化情况。通过半胱天冬酶激活分析以及对聚（ADP-核糖）聚合酶（PARP）裂解进行分子检测来评估凋亡情况。将射频与分化剂视黄酸或凋亡诱导剂喜树碱联合暴露，以测试电磁场与化学试剂之间可能存在的干扰。总体而言，我们的数据表明，长达72小时的900 MHz射频暴露不会在神经母细胞瘤细胞系的三种主要细胞活动中诱导显著改变。
