Markkanen Ari, Penttinen Piia, Naarala Jonne, Pelkonen Jukka, Sihvonen Ari-Pekka, Juutilainen Jukka
Department of Environmental Sciences, University of Kuopio, Kuopio, Finland.
Bioelectromagnetics. 2004 Feb;25(2):127-33. doi: 10.1002/bem.10167.
The aim of this study was to investigate whether radiofrequency (RF) electromagnetic field (EMF) exposure affects cell death processes of yeast cells. Saccharomyces cerevisiae yeast cells of the strains KFy417 (wild-type) and KFy437 (cdc48-mutant) were exposed to 900 or 872 MHz RF fields, with or without exposure to ultraviolet (UV) radiation, and incubated simultaneously with elevated temperature (+37 degrees C) to induce apoptosis in the cdc48-mutated strain. The RF exposure was carried out in a special waveguide exposure chamber where the temperature of the cell cultures can be precisely controlled. Apoptosis was analyzed using the annexin V-FITC method utilizing flow cytometry. Amplitude modulated (217 pulses per second) RF exposure significantly enhanced UV induced apoptosis in cdc48-mutated cells, but no effect was observed in cells exposed to unmodulated fields at identical time-average specfic absorption rates (SAR, 0.4 or 3.0 W/kg). The findings suggest that amplitude modulated RF fields, together with known damaging agents, can affect the cell death process in mutated yeast cells. Bioelectromagnetics 25:127-133, 2004.
本研究的目的是调查射频（RF）电磁场（EMF）暴露是否会影响酵母细胞的细胞死亡过程。将KFy417（野生型）和KFy437（cdc48突变型）菌株的酿酒酵母细胞暴露于900或872 MHz的射频场中，同时暴露或不暴露于紫外线（UV）辐射，并在升高的温度（+37摄氏度）下孵育以诱导cdc48突变菌株发生凋亡。射频暴露在一个特殊的波导暴露室中进行，在该暴露室中可以精确控制细胞培养物的温度。使用膜联蛋白V-FITC方法并利用流式细胞术分析凋亡情况。在相同的时间平均比吸收率（SAR，0.4或3.0 W/kg）下，调幅（每秒217个脉冲）的射频暴露显著增强了紫外线诱导的cdc48突变细胞的凋亡，但在暴露于未调制场的细胞中未观察到影响。这些发现表明，调幅射频场与已知的损伤因子一起，可影响突变酵母细胞的细胞死亡过程。《生物电磁学》25:127 - 133，2004年。
