一种用于通过肌红蛋白-鲁米诺化学发光系统测定一氧化碳的灵敏化学发光方法。

A sensitive chemiluminescence procedure for the determination of carbon monoxide with myoglobin-luminol chemiluminescence system.

作者信息

Xie Xiaofeng, He Xili, Song Zhenghua

机构信息

Department of Chemistry, Shaanxi Key Laboratory of Physico-Inorganic Chemistry, Northwest University, Xi'an, 710069, China.

出版信息

Appl Spectrosc. 2007 Jul;61(7):706-10. doi: 10.1366/000370207781393398.

DOI:10.1366/000370207781393398

PMID:17697464

Abstract

A novel chemiluminescence method combined with the flow injection technique for the determination of carbon monoxide is presented in this paper. The chemiluminescence signal based on the reaction between myoglobin and luminol in an alkaline medium was remarkably enhanced by carbon monoxide. The enhanced chemiluminescence intensity was linear with carbon monoxide concentration in the range from 0.01 to 10.0 pmol.L(-1), and the detection limit was 3x10(-3) pmol.L(-1) (3sigma). The whole process, including sampling and washing, could be completed in 0.5 min with a relative standard deviation of less than 4.0%. The proposed method was applied successfully in the assay of carbon monoxide in human serum and artificial water samples without any pretreatment procedure.

摘要

本文提出了一种结合流动注射技术的新型化学发光法用于测定一氧化碳。在碱性介质中，基于肌红蛋白与鲁米诺之间反应的化学发光信号被一氧化碳显著增强。增强的化学发光强度与一氧化碳浓度在0.01至10.0 pmol·L⁻¹范围内呈线性关系，检测限为3×10⁻³ pmol·L⁻¹（3σ）。整个过程，包括采样和清洗，可在0.5分钟内完成，相对标准偏差小于4.0%。所提出的方法无需任何预处理程序，已成功应用于人血清和人工水样中一氧化碳的测定。