Tabano Silvia, Alvino Gioia, Antonazzo Patrizio, Grati Francesca R, Miozzo Monica, Cetin Irene
Department of Medicine Surgery and Dentistry, University of Milano, Italy.
Pediatr Res. 2006 Feb;59(2):250-3. doi: 10.1203/01.pdr.0000199441.62045.a1.
Intrauterine growth restriction (IUGR) is associated with reduced placental supply of nutrients to the fetus. Lipoprotein lipase (LPL) mediates the hydrolysis of triglycerides from maternal lipoproteins to obtain fatty acids. Here, we tested the hypothesis that placental LPL gene expression level is altered in pregnancies complicated by IUGR. To this purpose, 28 IUGR fetuses were identified during pregnancy and divided in two groups: 7 M-IUGR ["mild" IUGR, with normal umbilical artery pulsatility index (PI)] and 21 S-IUGR ("severe" IUGR, with abnormal PI). Moreover, 10 out of 28 IUGR pregnancies were associated with preeclampsia. Controls were 19 normal pregnancies delivering appropriate for gestational age (AGA) fetuses. Relative real-time quantification of LPL was carried out in RNA from placental chorionic villi by the DeltaDeltaCt method, using beta-actin as normalizing gene. Placental LPL mRNA expression levels were significantly higher in IUGR than in AGA. In particular, significantly higher values were observed in S-IUGR, independent from the concomitant association with preeclampsia. No significant relationship was observed between placental LPL mRNA expression levels or gestational age. In conclusion, placental LPL mRNA gene expression is increased in severe IUGR, characterized by enhanced vascular placental resistances and alterations of placental nutrient transport.
宫内生长受限（IUGR）与胎盘向胎儿供应营养物质减少有关。脂蛋白脂肪酶（LPL）介导母体脂蛋白中甘油三酯的水解以获取脂肪酸。在此，我们检验了这样一个假设：在并发IUGR的妊娠中胎盘LPL基因表达水平会发生改变。为此，在孕期识别出28例IUGR胎儿并将其分为两组：7例M-IUGR ["轻度"IUGR，脐动脉搏动指数（PI）正常]和21例S-IUGR ["重度"IUGR，PI异常]。此外，28例IUGR妊娠中有10例与子痫前期有关。对照组为19例分娩适于胎龄（AGA）胎儿的正常妊娠。采用DeltaDeltaCt法，以β-肌动蛋白作为标准化基因，对胎盘绒毛膜RNA中的LPL进行相对实时定量分析。IUGR组胎盘LPL mRNA表达水平显著高于AGA组。特别是，S-IUGR组观察到显著更高的值，且与子痫前期的合并存在无关。胎盘LPL mRNA表达水平与胎龄之间未观察到显著关系。总之，在以胎盘血管阻力增加和胎盘营养物质转运改变为特征的重度IUGR中，胎盘LPL mRNA基因表达增加。
