Vimont A, Vernozy-Rozand C, Delignette-Muller M-L
Unité de Microbiologie Alimentaire et Prèvisionnelle, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
Lett Appl Microbiol. 2006 Feb;42(2):102-8. doi: 10.1111/j.1472-765X.2005.01818.x.
To review and characterize the enrichment protocols used for detecting all Shiga-Toxin producing Escherichia coli (STEC) from different matrices.
Firstly, the frequency distribution of the factors characterizing the enrichment protocols is described; secondly, a multiple correspondence analysis is performed to display profiles of association of these factors, and thirdly, published results concerning the relative performances of the protocols are summarized. Trypticase Soy Broth (TSB) is reported as the most frequently used enrichment broth. More often, one antibiotic is added in enrichment broths and these broths are incubated for a duration of 16-24 h at 35-37 degrees C. It also appears that the incubation temperature does not seem to be related to the type of serogroup looked for and that antibiotics are used regardless of the matrix analysed. Finally, results relating to the enrichment protocol efficacy are rare and differ from one study to another.
Statistical studies must be conducted so as to assess the efficacy of the main enrichment protocols investigated in this study.
This study reviews the most commonly used enrichment protocols and highlights the lack of results as to their relative efficacy.
回顾并描述用于从不同基质中检测所有产志贺毒素大肠杆菌（STEC）的富集方案。
首先，描述表征富集方案的因素的频率分布；其次，进行多重对应分析以展示这些因素的关联概况；第三，总结有关这些方案相对性能的已发表结果。胰蛋白胨大豆肉汤（TSB）被报道为最常用的富集肉汤。更常见的是，在富集肉汤中添加一种抗生素，这些肉汤在35 - 37摄氏度下孵育16 - 24小时。还发现孵育温度似乎与所寻找的血清群类型无关，并且无论分析的基质如何都使用抗生素。最后，关于富集方案有效性的结果很少，且不同研究之间存在差异。
必须进行统计研究以评估本研究中所研究的主要富集方案的有效性。
本研究回顾了最常用的富集方案，并强调了关于其相对有效性缺乏结果的情况。
