Rosman-Urbach Maya, Niv Yaron, Birk Yehudith, Morgenstern Sara, Schwartz Betty
Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel.
Br J Nutr. 2006 Jan;95(1):188-95. doi: 10.1079/bjn20051624.
UC (ulcerative colitis) patients have an increased risk of developing colorectal cancer compared with the normal population. The cause underlying this higher risk is not fully defined but includes nutritional and environmental factors concomitant with genetic alterations. We aimed to evaluate genetic stability in the colonic tissue of UC patients in clinical remission compared with the healthy population, and to establish a possible correlation between nutritional habits and these molecular assessments. UC patients (n 42) and healthy controls (n 37) participated in the study. All participants were histopathologically and medically diagnosed. Participants completed five separate 7 d dietary records, food-frequency questionnaires and validated 24 h recalls for nutritional assessment. The extent of chromosome 17 loss and the calculated chromosome index was determined in colon tissue biopsies by fluorescence in situ hybridisation. Correlations between the molecular and nutritional assessments were performed using Pearson's correlation coefficients. Significant differences in the nutritional intake of total fat (65 (SD 15) v. 89 (SD 25) g), cholesterol (330 (SD 168) v. 464 (SD 177) mg), dietary fibre (32 (SD 4.7) v. 9 (SD 4) g), vitamin A (1009 (SD 209) v. 506 (SD 204) microg), vitamin C (308 (SD 108) v. 72 (SD 53) mg) and folic acid (412 (SD 89 microg) v. 187 (SD 107)) were recorded for UC patients compared with controls. Significant correlations were found for the consumption of different food groups and the chromosome index for chromosome 17. The results of our study suggest that the nutritional habits adopted by UC patients during clinical remission may affect key cellular components of the colonic tissue, inducing a high degree of aneuploidy and genetic instability, and probably affecting the development of colon cancer.
与正常人群相比，溃疡性结肠炎（UC）患者患结直肠癌的风险增加。这种较高风险背后的原因尚未完全明确，但包括与基因改变相伴的营养和环境因素。我们旨在评估临床缓解期UC患者结肠组织与健康人群相比的基因稳定性，并确定饮食习惯与这些分子评估之间可能存在的相关性。UC患者（n = 42）和健康对照者（n = 37）参与了该研究。所有参与者均经过组织病理学和医学诊断。参与者完成了五份独立的7天饮食记录、食物频率问卷，并通过有效的24小时回顾进行营养评估。通过荧光原位杂交确定结肠组织活检中17号染色体缺失的程度和计算出的染色体指数。使用Pearson相关系数进行分子评估与营养评估之间的相关性分析。记录显示，与对照组相比，UC患者在总脂肪（65（标准差15）对89（标准差25）克）、胆固醇（330（标准差168）对464（标准差177）毫克）、膳食纤维（32（标准差4.7）对9（标准差4）克）、维生素A（1009（标准差209）对506（标准差204）微克）、维生素C（308（标准差108）对72（标准差53）毫克）和叶酸（412（标准差89微克）对187（标准差107））的营养摄入方面存在显著差异。发现不同食物组的摄入量与17号染色体的染色体指数之间存在显著相关性。我们的研究结果表明，UC患者在临床缓解期所采用的饮食习惯可能会影响结肠组织的关键细胞成分，导致高度的非整倍性和基因不稳定，并可能影响结肠癌的发生发展。
