Lau S K P, Ng K H L, Woo P C Y, Yip K-T, Fung A M Y, Woo G K S, Chan K-M, Que T-L, Yuen K-Y
Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong.
J Clin Pathol. 2006 Feb;59(2):219-22. doi: 10.1136/jcp.2004.025247.
Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
以完整的16S核糖体RNA(rRNA)基因测序作为金标准,采用MicroSeq 500 16S rDNA细菌鉴定系统对从血培养物中分离出的20株非重复厌氧革兰氏阳性杆菌进行分析。MicroSeq系统成功鉴定出了20株分离株中的13株。分别有4株和3株分离株在属和种水平上被错误鉴定。尽管MicroSeq 500 16S rDNA细菌鉴定系统优于另外三种同样经过评估的市售鉴定系统,但其数据库仍需扩展,以便准确鉴定厌氧革兰氏阳性杆菌。