Simmon Keith E, Croft Ann C, Petti Cathy A
ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
J Clin Microbiol. 2006 Dec;44(12):4400-6. doi: 10.1128/JCM.01364-06. Epub 2006 Oct 18.
Laboratories often receive clinical isolates for bacterial identification that have ambiguous biochemical profiles by conventional testing. With the emergence of 16S rRNA gene sequencing as an identification tool, we evaluated the usefulness of SmartGene IDNS, a 16S rRNA sequence database and software program for microbial identification. Identification by conventional methods of a diverse group of bacterial clinical isolates was compared with gene sequences interrogated by the SmartGene and MicroSeq databases. Of 300 isolates, SmartGene identified 295 (98%) to the genus level and 262 (87%) to the species level, with 5 (2%) being inconclusive. MicroSeq identified 271 (90%) to the genus level and 223 (74%) to the species level, with 29 (10%) being inconclusive. SmartGene and MicroSeq agreed on the genus for 233 (78%) isolates and the species for 212 (71%) isolates. Conventional methods identified 291 (97%) isolates to the genus level and 208 (69%) to the species level, with 9 (3%) being inconclusive. SmartGene, MicroSeq, and conventional identifications agreed for 193 (64%) of the results. Twenty-seven microorganisms were not represented in MicroSeq, compared to only 2 not represented in SmartGene. Overall, SmartGene IDNS provides comprehensive and accurate identification of a diverse group of bacteria and has the added benefit of being a user-friendly program that can be modified to meet the unique needs of clinical laboratories.
实验室经常收到用于细菌鉴定的临床分离株，这些分离株通过传统检测方法得出的生化特征不明确。随着16S rRNA基因测序作为一种鉴定工具的出现，我们评估了SmartGene IDNS的实用性，它是一个用于微生物鉴定的16S rRNA序列数据库和软件程序。将一组不同的细菌临床分离株通过传统方法进行的鉴定与SmartGene和MicroSeq数据库查询的基因序列进行了比较。在300株分离株中，SmartGene在属水平上鉴定出295株（98%），在种水平上鉴定出262株（87%），有5株（2%）结果不确定。MicroSeq在属水平上鉴定出271株（90%），在种水平上鉴定出223株（74%），有29株（10%）结果不确定。SmartGene和MicroSeq在233株（78%）分离株的属鉴定上达成一致，在212株（71%）分离株的种鉴定上达成一致。传统方法在属水平上鉴定出291株（97%）分离株，在种水平上鉴定出208株（69%），有9株（3%）结果不确定。SmartGene、MicroSeq和传统鉴定结果在193例（64%）中达成一致。MicroSeq中未涵盖27种微生物，而SmartGene中未涵盖的只有2种。总体而言，SmartGene IDNS能对多种细菌进行全面、准确的鉴定，并且还有一个额外的优点，即它是一个用户友好的程序，可以进行修改以满足临床实验室的独特需求。
