Fidan Isil, Yüksel Sevgi, Kalkanci Ayse, Imir Turgut, Kustimur Semra
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey.
Mem Inst Oswaldo Cruz. 2005 Dec;100(8):883-7. doi: 10.1590/s0074-02762005000800010. Epub 2006 Jan 20.
Type I diabetes mellitus (insulin-dependent DM = IDDM) is a chronic disease characterized by specific destruction of pancreatic beta cells, resulting in an absolute lack of insulin. Immune mechanisms, genetic susceptibility, and environmental factors are all implicated in the pathogenesis of Type 1 diabetes. This study was aimed at determining the efficiency of cytokines, natural killer (NK) cells in the pathophysiology of IDDM. Therefore, we evaluated the plasma levels of cytokines by specific enzyme-linked immunosorbent assay (ELISA) and the cytotoxicity activity of NK cells by anti-candididal index in rats with type I diabetes. We found that the cytotoxicity activity of NK cells in IDDM groups significantly decreased compared to the control groups. The levels of interferon-gamma (IFN-gamma) in IDDM groups were slightly higher than in healthy controls. These results indicate that the changes of T H1 type cytokines such as IFN-gamma and NK cell activity can play a role in the etiology of IDDM. The data may provide new strategies for the treatment of IDDM.
1型糖尿病（胰岛素依赖型糖尿病 = IDDM）是一种以胰腺β细胞特异性破坏为特征的慢性疾病，导致胰岛素绝对缺乏。免疫机制、遗传易感性和环境因素均与1型糖尿病的发病机制有关。本研究旨在确定细胞因子、自然杀伤（NK）细胞在IDDM病理生理学中的作用。因此，我们通过特异性酶联免疫吸附测定（ELISA）评估了细胞因子的血浆水平，并通过抗念珠菌指数评估了1型糖尿病大鼠NK细胞的细胞毒性活性。我们发现，与对照组相比，IDDM组NK细胞的细胞毒性活性显著降低。IDDM组的干扰素-γ（IFN-γ）水平略高于健康对照组。这些结果表明，IFN-γ等TH1型细胞因子的变化和NK细胞活性可能在IDDM的病因中起作用。这些数据可能为IDDM的治疗提供新策略。
