Sneyd J, Tsaneva-Atanasova K, Reznikov V, Bai Y, Sanderson M J, Yule D I
Department of Mathematics, University of Auckland, Auckland, New Zealand.
Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1675-80. doi: 10.1073/pnas.0506135103. Epub 2006 Jan 30.
In some cell types, oscillations in the concentration of free intracellular calcium ([Ca2+]) are accompanied by oscillations in the concentration of inositol 1,4,5-trisphosphate ([IP3]). However, in most cell types it is still an open question as to whether oscillations in [IP3] are necessary for Ca2+ oscillations in vivo, or whether they merely follow passively. Using a wide range of models, we show that the response to an artificially applied pulse of IP3 can be used to distinguish between these two cases. Hence, we show that muscarinic receptor-mediated, long-period Ca2+ oscillations in pancreatic acinar cells depend on [IP3] oscillations, whereas short-period Ca2+ oscillations in airway smooth muscle do not.
在某些细胞类型中，细胞内游离钙（[Ca2+]）浓度的振荡伴随着肌醇1,4,5-三磷酸（[IP3]）浓度的振荡。然而，在大多数细胞类型中，[IP3]的振荡在体内对于Ca2+振荡是否必要，或者它们是否仅仅是被动跟随，仍然是一个悬而未决的问题。通过使用多种模型，我们表明对人工施加的IP3脉冲的反应可用于区分这两种情况。因此，我们表明胰腺腺泡细胞中由毒蕈碱受体介导的长周期Ca2+振荡依赖于[IP3]振荡，而气道平滑肌中的短周期Ca2+振荡则不依赖。
