Sadano H, Inoue M, Taniguchi S
Department of Molecular and Cellular Biology, Kyushu University.
Jpn J Cancer Res. 1992 Jun;83(6):625-30. doi: 10.1111/j.1349-7006.1992.tb00135.x.
We previously reported on the altered expression of a third actin in mouse-B16 melanoma associated with malignant progression. While further investigating the relationship of cytoskeletal proteins to malignancy, we found that the expression of vinculin was higher in weakly metastatic B16-F1 cells than in highly metastatic B16-F10 cells. By Northern blot analysis, the mRNA expression of vinculin in B16-F1 was also shown to be higher than in B16-F10. Immunofluorescence staining showed a clear dotted distribution of vinculin in B16-F1, but only a weak and diffuse distribution in B16-F10. The dotted distribution tended to be larger in B16-F1 and when cultured on Matrigel and fibronectin than on laminin and type IV collagen. An alteration in the expression of vinculin was also observed in other cell systems. Vinculin was detected in both normal 3Y1 and in relatively weakly malignant transformed 3Y1 cell lines, while vinculin was either scarcely detected or not detected at all in more malignant cell lines. These results suggest that the suppression of vinculin is closely related to malignant progression in both the B16-melanoma and 3Y1 cell systems.
我们之前报道过，在与恶性进展相关的小鼠B16黑色素瘤中，第三种肌动蛋白的表达发生了改变。在进一步研究细胞骨架蛋白与恶性肿瘤的关系时，我们发现黏着斑蛋白在低转移性B16-F1细胞中的表达高于高转移性B16-F10细胞。通过Northern印迹分析，还显示B16-F1中黏着斑蛋白的mRNA表达高于B16-F10。免疫荧光染色显示，黏着斑蛋白在B16-F1中呈明显的点状分布，但在B16-F10中仅呈微弱的弥漫性分布。在B16-F1中，当在基质胶和纤连蛋白上培养时，点状分布往往比在层粘连蛋白和IV型胶原上培养时更大。在其他细胞系统中也观察到了黏着斑蛋白表达的改变。在正常的3Y1细胞和恶性程度相对较低的转化3Y1细胞系中均检测到了黏着斑蛋白，而在恶性程度更高的细胞系中，黏着斑蛋白要么几乎检测不到，要么根本检测不到。这些结果表明，在B16黑色素瘤和3Y1细胞系统中，黏着斑蛋白的抑制与恶性进展密切相关。
