扫描电化学显微镜。56. 探测含有三联吡啶钌（Ⅱ）（Ru(bpy)₃²⁺）的单个巨型脂质体的内部和外部。

Scanning electrochemical microscopy. 56. Probing outside and inside single giant liposomes containing Ru(bpy)32+.

作者信息

Zhan Wei, Bard Allen J

机构信息

Department of Chemistry and Biochemistry, University of Texas, Austin, 78712, USA.

出版信息

Anal Chem. 2006 Feb 1;78(3):726-33. doi: 10.1021/ac051290a.

DOI:10.1021/ac051290a

PMID:16448045

Abstract

Giant liposomes containing Ru(bpy)(3)(2+) (bpy = 2,2'-bipyridine) were prepared as model systems for biomembranes and cells and studied by scanning electrochemical microscopy (SECM). Conical carbon fiber tips of submicrometer size were used to approach, image, and puncture individual liposomes immobilized on glass substrates. SECM images of the liposomes were obtained, and the leakage of Ru(bpy)(3)(2+) through the lipid membrane was probed. The tip was also pushed into liposomes and characteristic breakthrough transients, corresponding to liposomes with different compartmental configurations, were obtained. Voltammograms were obtained with the tip inside a single liposome after breaking through the membrane, and the influx of mediator and efflux of encapsulant after puncture could be observed.

摘要

制备了含有Ru(bpy)(3)(2+)（bpy = 2,2'-联吡啶）的巨型脂质体，作为生物膜和细胞的模型系统，并通过扫描电化学显微镜（SECM）进行研究。使用亚微米尺寸的锥形碳纤维尖端来接近、成像和穿刺固定在玻璃基板上的单个脂质体。获得了脂质体的SECM图像，并探测了Ru(bpy)(3)(2+)通过脂质膜的泄漏情况。尖端还被推进脂质体中，得到了与具有不同隔室构型的脂质体相对应的特征性突破瞬变。在穿透膜后，在单个脂质体内用尖端获得了伏安图，并且可以观察到穿刺后介质的流入和封装剂的流出。