Sun Peng, Laforge François O, Abeyweera Thushara P, Rotenberg Susan A, Carpino James, Mirkin Michael V
Department of Chemistry and Biochemistry, Queens College-City University of New York, Flushing, NY 11367, USA.
Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):443-8. doi: 10.1073/pnas.0711075105. Epub 2008 Jan 4.
There is a significant current interest in development of new techniques for direct characterization of the intracellular redox state and high-resolution imaging of living cells. We used nanometer-sized amperometric probes in combination with the scanning electrochemical microscope (SECM) to carry out spatially resolved electrochemical experiments in cultured human breast cells. With the tip radius approximately 1,000 times smaller than that of a cell, an electrochemical probe can penetrate a cell and travel inside it without apparent damage to the membrane. The data demonstrate the possibility of measuring the rate of transmembrane charge transport and membrane potential and probing redox properties at the subcellular level. The same experimental setup was used for nanoscale electrochemical imaging of the cell surface.
当前，人们对开发用于直接表征细胞内氧化还原状态和活细胞高分辨率成像的新技术有着浓厚的兴趣。我们将纳米级安培探针与扫描电化学显微镜（SECM）结合使用，在培养的人乳腺细胞中进行空间分辨电化学实验。由于探针尖端半径比细胞小约1000倍，电化学探针可以穿透细胞并在其中移动，而不会对细胞膜造成明显损伤。数据表明，在亚细胞水平上测量跨膜电荷传输速率、膜电位以及探测氧化还原特性是可能的。相同的实验装置用于细胞表面的纳米级电化学成像。
