Bhattacharyya Arpita, Klapperich Catherine M
Department of Biomedical Engineering, Boston University, Massachusetts 02215, USA.
Anal Chem. 2006 Feb 1;78(3):788-92. doi: 10.1021/ac051449j.
A polymeric microfluidic device for solid-phase extraction (SPE)-based isolation of nucleic acids is demonstrated. The plastic chip can function as a disposable sample preparation system for different biological and diagnostic applications. The chip was fabricated in a cyclic polyolefin by hot-embossing with a master mold. The solid phase consisted of a porous monolithic polymer column impregnated with silica particles. The extraction was achieved due to the binding of nucleic acids to the silica particles in the monolith. The solid phase was formed within the channels of the device by in situ photoinitiated polymerization of a mixture of methacrylate and dimethacrylate monomers, UV-sensitive free-radical initiator, and porogenic solvents. The channel surfaces were pretreated via photografting to covalently attach the monolith to the channel walls. The solid phase prepared by this method allowed for successful extraction and elution of nucleic acids in the polymeric microchip.
展示了一种基于固相萃取（SPE）的用于分离核酸的聚合物微流控装置。该塑料芯片可作为用于不同生物和诊断应用的一次性样品制备系统。该芯片是通过用母模热压印在环状聚烯烃中制造的。固相由浸渍有二氧化硅颗粒的多孔整体聚合物柱组成。由于核酸与整体中的二氧化硅颗粒结合而实现提取。通过甲基丙烯酸酯和二甲基丙烯酸酯单体、紫外线敏感自由基引发剂和成孔溶剂的混合物的原位光引发聚合在装置的通道内形成固相。通道表面通过光接枝进行预处理，以使整体共价连接到通道壁上。通过这种方法制备的固相能够在聚合物微芯片中成功提取和洗脱核酸。
