Katayama Shigeru, Xu Xueming, Fan Ming Z, Mine Yoshinori
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
J Agric Food Chem. 2006 Feb 8;54(3):773-8. doi: 10.1021/jf052280d.
The protective effects of hen egg yolk phosvitin phosphopeptides (PPPs) against hydrogen peroxide (H2O2)-induced oxidative stress were evaluated in an in vitro assay using human intestinal epithelial cells. Caco-2 cells were stimulated with 1 mM H2O2 for 6 h, and the secretion of IL-8, a proinflammatory mediator, was determined by ELISA as a biomarker of oxidative stress. The inhibition of H2O2-induced IL-8 secretion from Caco-2 cells was observed by pretreatment for 2 h with PPPs, but not with phosvitin. PPPs also suppressed the formation of malondialdehyde in H2O2-treated Caco-2 cells. Furthermore, intracellular glutathione levels and glutathione reductase activity were elevated by the addition of PPPs. The protective effects of PPPs against H2O2-induced oxidative stress were almost the same as that of glutathione, and PPPs with a high content of phosphorus exhibited higher protective activity than PPPs without phosphorus; however, phosphoserine itself did not show any significant antioxidative stress activity. These findings suggest that oligophosphopeptides from hen egg yolk phosvitin possess novel antioxidative activity against oxidative stress in intestinal epithelial cells and that phosphorus and peptide structure seem to have a key role in the activity.
在一项使用人肠上皮细胞的体外试验中，评估了鸡蛋黄卵黄高磷蛋白磷酸肽（PPPs）对过氧化氢（H2O2）诱导的氧化应激的保护作用。用1 mM H2O2刺激Caco-2细胞6小时，并通过酶联免疫吸附测定法（ELISA）测定促炎介质白细胞介素-8（IL-8）的分泌，将其作为氧化应激的生物标志物。通过用PPPs预处理2小时观察到对H2O2诱导的Caco-2细胞IL-8分泌的抑制作用，但用卵黄高磷蛋白预处理则未观察到该作用。PPPs还抑制了H2O2处理的Caco-2细胞中丙二醛的形成。此外，添加PPPs可提高细胞内谷胱甘肽水平和谷胱甘肽还原酶活性。PPPs对H2O2诱导的氧化应激的保护作用与谷胱甘肽的保护作用几乎相同，且磷含量高的PPPs比无磷的PPPs表现出更高的保护活性；然而，磷酸丝氨酸本身并未显示出任何显著的抗氧化应激活性。这些发现表明，来自鸡蛋黄卵黄高磷蛋白的寡聚磷酸肽对肠上皮细胞的氧化应激具有新的抗氧化活性，并且磷和肽结构似乎在该活性中起关键作用。
