使用纳米晶羟基磷灰石和人冻干骨移植材料进行骨再生的组织学和组织形态计量学评估：一项在兔身上的实验研究。
Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft : An experimental study in rabbit.
作者信息
Sadeghi Rokhsareh, Najafi Mohammad, Semyari Hassan, Mashhadiabbas Fatemeh
机构信息
Department of Periodontics, Faculty of Dentistry, Shahed University, 37, Italia St., Vesal Ave., Tehran, 1417755351, Iran.
Department of Periodontics, Faculty of Dentistry, Golestan University of Medical Sciences, Gorgan, Golestan, Iran.
出版信息
J Orofac Orthop. 2017 Mar;78(2):144-152. doi: 10.1007/s00056-016-0067-8. Epub 2017 Jan 27.
PURPOSE
Bone regeneration is an important concern in periodontal treatment and implant dentistry. Different biomaterials and surgical techniques have been used for this purpose. The aim of the present study was to compare the effect of nanocrystalline hydroxyapatite and human freeze-dried bone graft (FDBG) in regeneration of rabbit calvarium bony defects by histologic and histomorphometric evaluation.
METHODS
In this experimental study, three similar defects, measuring 8 mm in diameter, were created in the calvaria of 16 white New Zealand rabbits. Two defects were filled with FDBG and nanocrystalline hydroxyapatite silica gel, while the other one remained unfilled to be considered as control. All the defects were covered with collagen membranes. During the healing period, two animals perished; so 14 rabbits were divided into two groups: half of them were euthanized after 6 weeks of healing and the other half after 12 weeks. The specimens were subjected to histologic and histomorphometric examinations for assessment of the following variables: percentage of bone formation and residual graft material, inflammation scores, patterns of bone formation and type of newly formed bone.
RESULTS
The percentages of new bone formation after 6 weeks were 14.22 ± 7.85, 21.57 ± 6.91, and 20.54 ± 10.07% in FDBG, NanoBone, and control defects. These values were 27.54 ± 20.19, 23.86 ± 6.27, and 26.48 ± 14.18% in 12-week specimens, respectively. No significant differences were found in the amount of bone formation between the groups. With regard to inflammation, the control and NanoBone groups showed significantly less inflammation compared to FDBG at the 6-week healing phase (P = 0.04); this difference was not significant in the 12-week specimens.
CONCLUSIONS
Based on the results of this experimental study, both NanoBone and FDBG exhibited a similar effect on bone formation.
目的
骨再生是牙周治疗和种植牙科领域的一个重要关注点。为此人们使用了不同的生物材料和手术技术。本研究的目的是通过组织学和组织形态计量学评估，比较纳米晶羟基磷灰石和人冻干骨移植材料（FDBG）对兔颅骨骨缺损再生的影响。
方法
在这项实验研究中，在16只白色新西兰兔的颅骨上制造了3个直径为8毫米的类似缺损。两个缺损分别用FDBG和纳米晶羟基磷灰石硅胶填充，另一个缺损不填充作为对照。所有缺损均用胶原膜覆盖。在愈合期，有2只动物死亡；因此，14只兔子被分为两组：其中一半在愈合6周后安乐死，另一半在12周后安乐死。对标本进行组织学和组织形态计量学检查，以评估以下变量：骨形成百分比和残余移植材料、炎症评分、骨形成模式和新形成骨的类型。
结果
6周后，FDBG、纳米骨和对照缺损处新骨形成的百分比分别为14.22±7.85%、21.57±6.91%和20.54±10.07%。在12周的标本中，这些值分别为27.54±20.19%、23.86±6.27%和26.48±14.18%。各组之间在骨形成量上未发现显著差异。关于炎症，在6周愈合期，对照组和纳米骨组的炎症明显少于FDBG组（P = 0.04）；在12周的标本中，这种差异不显著。
结论
基于这项实验研究的结果，纳米骨和FDBG对骨形成表现出相似的效果。
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