Pompa G, Arioli F, Fracchiolla M L, Rossi C A Sgoifo, Bassini A L, Stella S, Biondi P A
Department of Veterinary Science and Technology for Food Safety, Faculty of Veterinary Medicine, University of Milan, via Celoria 10, I-20133, Milan, Italy.
Food Addit Contam. 2006 Feb;23(2):126-32. doi: 10.1080/02652030500442508.
Conflicting findings regarding the boldenone content of bovine faeces suggest it may be synthesized de novo in emitted faeces. We tested this hypothesis by analysing uncontaminated urine, fresh and various forms of dried faeces from 10 calves (not given boldenone) by liquid chromatography/tandem mass spectrometry for 17alpha- and 17beta-boldenone (alpha and beta BOL); 1,4-androstadiene-3,17-dione (ADD); 4-androstene-3,17-dione (AED), testosterone (T) and epitestosterone (ET). Urine contained no alpha BOL, beta BOL or ADD. The analysed substances were variably present in the rectal faeces, and at generally higher levels in faeces scraped from skin or stall floor. In pooled rectal faeces naturally dried for 13 days, alpha BOL, ADD, AED and ET levels were extremely high (much higher than accounted for by increases due to drying), and beta BOL and T were absent. It is concluded that de novo synthesis of alpha BOL and metabolites occurs naturally in bovine faeces and only uncontaminated urine should be analysed for illegal boldenone.
关于牛粪中勃地酮含量的研究结果相互矛盾，这表明它可能在排出的粪便中重新合成。我们通过液相色谱/串联质谱法分析了10头犊牛（未给予勃地酮）的未受污染尿液、新鲜粪便和各种形式的干燥粪便，以检测17α-和17β-勃地酮（α和β BOL）、1,4-雄二烯-3,17-二酮（ADD）、4-雄烯-3,17-二酮（AED）、睾酮（T）和表睾酮（ET），从而验证这一假设。尿液中未检测到α BOL、β BOL或ADD。所分析的物质在直肠粪便中的含量各不相同，而从皮肤或畜栏地面刮取的粪便中含量通常更高。在自然干燥13天的混合直肠粪便中，α BOL、ADD、AED和ET的含量极高（远高于因干燥导致的增加量），且未检测到β BOL和T。结论是，α BOL及其代谢产物在牛粪中自然发生从头合成，并且仅应分析未受污染的尿液以检测非法的勃地酮。
