Ferretti G, Palleschi L, Marchiafava C, delli Quadri F, Fantozzi L, Ferranti C, Cammarata P, Macrì A, Montesissa C, Draisci R
Istituto Superiore di Sanità, Department of Food Safety and Veterinary Public Health, Roma, Italy.
Anal Chim Acta. 2007 Apr 25;589(2):269-74. doi: 10.1016/j.aca.2007.03.008. Epub 2007 Mar 12.
The residue profiles of boldenone (17beta-Bol), its epimer (17alpha-Bol) and the related compound androsta-1,4-diene-3,17-dione (ADD), were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in urine of male calves orally treated with boldenone, boldenone esters, and/or ADD. In all the experiments with the administered steroids residues of 17alpha-Bol decreased rapidly after end of treatment; detectable amounts of 17alpha-Bol were however noticed along the withdrawal observation period after end of treatment. Differently, residues of 17beta-Bol were detectable only shortly after administration. This in vivo research concerning oral treatments of cattle with boldenone related substances proves ADD to be a very active boldenone precursor in bovine animals.
采用液相色谱 - 串联质谱法（LC-MS/MS），对口服苯丙酸诺龙、苯丙酸诺龙酯和/或雄甾 - 1,4 - 二烯 - 3,17 - 二酮（ADD）的雄性犊牛尿液中的苯丙酸诺龙（17β - Bol）、其差向异构体（17α - Bol）及相关化合物ADD的残留情况进行了研究。在所有使用所给甾体的实验中，17α - Bol的残留量在治疗结束后迅速下降；不过，在治疗结束后的停药观察期内仍可检测到一定量的17α - Bol。不同的是，17β - Bol的残留仅在给药后短时间内可检测到。这项关于用苯丙酸诺龙相关物质对牛进行口服治疗的体内研究证明，ADD在牛体内是一种非常活跃的苯丙酸诺龙前体。
