Slobodkin A I, Tourova T P, Kostrikina N A, Lysenko A M, German K E, Bonch-Osmolovskaya E A, Birkeland N-K
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117 312 Moscow, Russia.
Institute of Physical Chemistry, Russian Academy of Sciences, Leninskiy prospect 31, 119 991 Moscow, Russia.
Int J Syst Evol Microbiol. 2006 Feb;56(Pt 2):369-372. doi: 10.1099/ijs.0.63694-0.
A moderately thermophilic, anaerobic bacterium (strain SB91T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91T were straight to slightly curved rods, 0.5-0.6 microm in diameter and 3.0-7.0 mum in length. Formation of endospores was not observed. The temperature range for growth was 26-62 degrees C, with an optimum at 50 degrees C. The pH range for growth was 5.5-9.5, with an optimum at pH 7.5-8.0. The substrates utilized by strain SB91T in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, L-valine and n-propanol. Carbohydrates were not utilized. Strain SB91T reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, L-valine or n-propanol as an electron donor. Strain SB91T reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91T survived after exposure to gamma-radiation at a dose of 5.4 kGy. The G+C content of the DNA of strain SB91T was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91T represents the sole species of a novel genus, Tepidimicrobium; the name Tepidimicrobium ferriphilum gen. nov., sp. nov. is proposed, with strain SB91T (=DSM 16624T=VKM B-2348T) as the type strain.
从俄罗斯布里亚特共和国巴尔古津山谷的一个淡水温泉中分离出一株嗜热厌氧细菌（菌株SB91T）。菌株SB91T的细胞呈直杆状至微弯杆状，直径0.5 - 0.6微米，长度3.0 - 7.0微米。未观察到芽孢形成。生长温度范围为26 - 62℃，最适温度为50℃。生长pH范围为5.5 - 9.5，最适pH为7.5 - 8.0。在9,10 - 蒽醌 - 2,6 - 二磺酸盐存在下，菌株SB91T利用的底物包括蛋白胨、胰蛋白胨、酪蛋白氨基酸、酵母提取物、牛肉提取物、酪蛋白水解物、丙氨酸加甘氨酸、丙氨酸加脯氨酸、L - 缬氨酸和正丙醇。不利用碳水化合物。菌株SB91T以蛋白胨、L - 缬氨酸或正丙醇作为电子供体时，可还原无定形氧化铁、柠檬酸铁（III）、乙二胺四乙酸铁（III）或次氮基三乙酸铁（III）。菌株SB91T可还原9,10 - 蒽醌 - 2,6 - 二磺酸盐、硫代硫酸盐、元素硫、富马酸盐和亚硒酸盐。菌株SB91T在5.4 kGy剂量的γ射线照射后仍能存活。菌株SB91T的DNA的G + C含量为33 mol%。对16S rRNA基因序列的分析表明，分离出的生物体属于梭菌属的第十二簇。根据其生理特性和系统发育分析结果，建议菌株SB91T代表一个新属——嗜温微菌属的唯一物种；提出新属名嗜温微菌属（Tepidimicrobium），新种名嗜铁嗜温微菌（Tepidimicrobium ferriphilum），模式菌株为菌株SB91T（=DSM 16624T=VKM B - 2348T）。
