Pollack J H, Ntamere A S, Neuhaus F C
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
J Gen Microbiol. 1992 May;138(5):849-59. doi: 10.1099/00221287-138-5-849.
Vesicles containing lipoteichoic acid (LTA) have been isolated from Lactobacillus casei ATCC 7469 grown in the presence of either benzylpenicillin or D-cycloserine. These cell wall antibiotics enhanced the rate of LTA and lipid secretion 6.7 times, whereas chloramphenicol inhibited their release. The formation of these vesicles from peripheral and septal wall regions did not appear to be the result of bacteriolysis. The vesicle composition of LTA and lipid was similar to that of the cytoplasmic membrane whereas the protein composition was dissimilar. The size of these vesicles ranged from 20 to 40 nm and the length of LTA ranged from 5 to 50 glycerol phosphate residues. The isolation of these vesicles provides a potential in vitro acceptor system for studying the D-alanylation of lipoteichoic acid.
已从在苄青霉素或D-环丝氨酸存在下生长的干酪乳杆菌ATCC 7469中分离出含有脂磷壁酸（LTA）的囊泡。这些细胞壁抗生素将LTA和脂质的分泌速率提高了6.7倍，而氯霉素则抑制它们的释放。这些囊泡从外周壁和隔膜壁区域形成似乎不是细菌溶解的结果。LTA和脂质的囊泡组成与细胞质膜相似，而蛋白质组成则不同。这些囊泡的大小范围为20至40纳米，LTA的长度范围为5至50个甘油磷酸残基。这些囊泡的分离为研究脂磷壁酸的D-丙氨酰化提供了一个潜在的体外受体系统。
