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从溶纤维丁酸弧菌中分离脂磷壁酸。

Isolation of lipoteichoic acids from Butyrivibrio fibrisolvens.

作者信息

Hewett M J, Wicken A J, Knox K W, Sharpe M E

出版信息

J Gen Microbiol. 1976 May;94(1):126-30. doi: 10.1099/00221287-94-1-126.

DOI:10.1099/00221287-94-1-126
PMID:819620
Abstract

Lipoteichoic acid (LTA) and deacylated lipoteichoic acid have been isolated from the bovine-rumen Gram-negative anaerobe Butyrivibrio fibrisolvens by phenol extraction. Lipoteichoic acid (21.8 mumol phosphorus/g cells) consisted of a conventional 1, 3-phosphodiester-linked chain of glycerol phosphate units joined covalently to a glycolipid. It was not substituted with glycosyl or D-alalyl ester groups. Deacylated lipoteichoic acid (57.5 mumol phosphorus/g cells) was similar in constitution but lacked fatty acid esters. Lipoteichoic acid reacted serologically with antisera to the glycerol phosphate backbone of known lipoteichoic acids. The presence of similar teichoic acid polymers has also been demonstrated in some other strains of B. fibrisolvens and this is of significance in demonstrating that teichoic acids can occur in Gram-negative bacteria.

摘要

通过苯酚萃取法从牛瘤胃革兰氏阴性厌氧菌溶纤维丁酸弧菌中分离出脂磷壁酸(LTA)和去酰基脂磷壁酸。脂磷壁酸(21.8微摩尔磷/克细胞)由一条常规的1,3-磷酸二酯键连接的甘油磷酸单元链组成,该链与一种糖脂共价连接。它没有被糖基或D-丙氨酰酯基团取代。去酰基脂磷壁酸(57.5微摩尔磷/克细胞)在组成上相似,但缺乏脂肪酸酯。脂磷壁酸与已知脂磷壁酸甘油磷酸主链的抗血清发生血清学反应。在其他一些溶纤维丁酸弧菌菌株中也证实了类似磷壁酸聚合物的存在,这对于证明磷壁酸可以存在于革兰氏阴性细菌中具有重要意义。

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引用本文的文献

1
Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains.Tn916、Tn916 delta E和pAM beta 1从粪肠球菌向溶纤维丁酸弧菌菌株的接合转移。
Appl Environ Microbiol. 1991 Sep;57(9):2703-9. doi: 10.1128/aem.57.9.2703-2709.1991.
2
Teichoic acids and lipids associated with the membrane of a Bacillus licheniformis mutant and the membrane lipids of the parental strain.与地衣芽孢杆菌突变体膜相关的磷壁酸和脂质以及亲本菌株的膜脂。
J Bacteriol. 1976 Oct;128(1):149-56. doi: 10.1128/jb.128.1.149-156.1976.
3
Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium.
溶纤维丁酸弧菌的超微结构:一种革兰氏阳性细菌。
J Bacteriol. 1977 Mar;129(3):1506-12. doi: 10.1128/jb.129.3.1506-1512.1977.