Burgyan J, Rubino L, Russo M
Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi, Bari, Italy.
J Gen Virol. 1991 Mar;72 ( Pt 3):505-9. doi: 10.1099/0022-1317-72-3-505.
Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low Mr RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from larger molecules. Computer-generated secondary structures of sequences surrounding recombination sites were extensive and stable and these sites occurred in interior or hairpin loops, thus providing a possible explanation for discontinuous RNA transcription and the formation of deletions in genomic RNA.
用体外合成的大花蕙兰环斑番茄病毒RNA接种克利夫兰烟草植株，之后通过汁液接种进行进一步传代。在第三次传代期间，出现了低分子量RNA种类，它们具有基因组RNA缺失突变体的特征。对其中几种缺陷干扰RNA的序列分析表明，可能存在从较大分子演变为较小分子的情况。重组位点周围序列的计算机生成二级结构广泛且稳定，这些位点出现在内部或发夹环中，从而为基因组RNA中的不连续RNA转录和缺失形成提供了一种可能的解释。
