Mlcochová Petra, Bystrický Slavomír, Steiner Bohumil, Machová Eva, Koós Miroslav, Velebný Vladimír, Krcmár Martin
CPN spol. s r.o., Dolní Dobrouc, Czech Republic.
Biopolymers. 2006 May;82(1):74-9. doi: 10.1002/bip.20461.
Several new biocompatible and degradable materials were prepared by chemical modification of sodium hyaluronate. The method of activation of hyaluronate by cyanogen bromide was used and subsequent reaction with nucleophile led to the formation of carbamate. This modification of hydroxyl groups of glycosaminoglycans preserves the carboxyl groups and retains properties of polyelectrolyte. This method affords derivatives easily and the reaction condition correlates with degree of substitution. The experimental results show the effect of reaction conditions (reaction time, ratio of reactants) and effect of substitution on biodegradability. The obtained materials were characterized by nuclear magnetic resonance and Fourier transform infrared spectroscopy.
通过对透明质酸钠进行化学修饰制备了几种新型的生物相容性和可降解材料。采用溴化氰活化透明质酸的方法，随后与亲核试剂反应生成氨基甲酸酯。这种对糖胺聚糖羟基的修饰保留了羧基并保持了聚电解质的性质。该方法易于得到衍生物，且反应条件与取代度相关。实验结果表明了反应条件（反应时间、反应物比例）以及取代对生物降解性的影响。所获得的材料通过核磁共振和傅里叶变换红外光谱进行了表征。
