European Molecular Biology Laboratory, Heidelberg, FRG.
EMBO J. 1982;1(2):255-61. doi: 10.1002/j.1460-2075.1982.tb01156.x.
The amino-acid sequence of the single-stranded DNA-binding protein of bacteriophage Pf1 and the nucleotide sequence of the corresponding gene have been determined. The protein has 144 amino acids and a molecular weight of 15 400; the gene consists of 435 nucleotides. The amino-acid sequence was determined by Edman degradation, carboxypeptidase A, B, and P digestion of intact protein and of peptides derived by chymotrypsin, Staphylococcus aureus V8 protease, and trypsin digestion. The nucleotide sequence was determined by the dideoxy method after random cloning of fragments of Pf1 DNA into M13. No sequence homology could be established between the amino-acid sequence of the DNA-binding protein of Pseudomonas aeruginosa-specific bacteriophage Pf1 and bacteriophage fd of Escherichia coli.
已测定 Pf1 噬菌体单链 DNA 结合蛋白的氨基酸序列和相应基因的核苷酸序列。该蛋白由 144 个氨基酸组成,分子量为 15400;基因由 435 个核苷酸组成。氨基酸序列通过 Edman 降解、完整蛋白的羧肽酶 A、B 和 P 消化以及糜蛋白酶、金黄色葡萄球菌 V8 蛋白酶和胰蛋白酶消化的肽段确定。核苷酸序列通过随机克隆 Pf1 DNA 片段到 M13 后,用双脱氧法确定。铜绿假单胞菌特异性噬菌体 Pf1 的 DNA 结合蛋白的氨基酸序列与大肠杆菌噬菌体 fd 之间没有序列同源性。