European Molecular Biology Laboratory, Postfach 10.2209, D-6900 Heidelberg, FRG.
EMBO J. 1982;1(8):995-8. doi: 10.1002/j.1460-2075.1982.tb01283.x.
Chloroplast DNA (cpDNA), containing 10% replicative molecules, was isolated 2 h after onset of the dark period from cultures of Euglena gracilis strain Z. The DNA was digested with the restriction enzymes PvuII, SalI, BamHI, or EcoRI. Fragments that contained intact replicative loops were measured to determine the position of replicated sequences in relation to the restriction enzyme sites. It was found that replication starts at a unique position near one of the palindromic sequences I(2) (Koller and Delius, 1982a) which is located upstream (with respect to the direction of rRNA transcription) of the AT-rich region of variable size (Jenni et al., 1981; Schlunegger et al., in preparation). In the majority of cases DNA synthesis proceeds unidirectionally away from this region for 5000 nucleotides before it starts in the other direction (in the same sense as the rRNA transcription) through the Z-region and the second palindromic sequence.
叶绿体 DNA（cpDNA）含有 10%的复制分子，在 Euglena gracilis Z 菌株的暗期开始后 2 小时从培养物中分离出来。用限制酶 PvuII、SalI、BamHI 或 EcoRI 消化 DNA。测量含有完整复制环的片段，以确定复制序列相对于限制酶位点的位置。结果发现，复制从靠近发夹序列 I(2)（Koller 和 Delius，1982a）的独特位置开始，该位置位于大小可变的富含 AT 区（Jenni 等人，1981；Schlunegger 等人，正在准备中）上游（相对于 rRNA 转录方向）。在大多数情况下，DNA 合成从该区域单向前进 5000 个核苷酸，然后在另一侧（与 rRNA 转录相同的方向）通过 Z 区和第二个发夹序列开始。
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