Orozco E M, Gray P W, Hallick R B
J Biol Chem. 1980 Nov 25;255(22):10991-6.
The organization of the genes in the rRNA coding region of Euglene gracilis Pringsheim strain Z chloroplast DNA has been determined. 125I labeled 16 S rRNA, 125I labeled 23 S rRNA, and [32P]tRNAs were hybridized to restriction nuclease fragments from cloned chloroplast DNA segments. The coding regions for 16 S and 23 S rRNAs have been located to within 1.75 and 3.1 kbp, respectively. Transfer rRNA genes are located in the 16 S to 23 S rRNA spacer region. Ribosomal RNAs and tRNAs are encoded on the same DNA strand in the 6.2 kbp tandemly repeated rRNA gene region. The order of genes, which is repeated three times in chloroplast DNA, is 16 S rRNA--tRNAS--23 S rRNA--5 S rRNA. Each set of genes is clustered in a region of 5.0 kbp. The organization and size of the genes is as expected if they are all part of a single transcription unit.
已经确定了纤细裸藻普林斯海姆菌株Z叶绿体DNA的rRNA编码区中基因的组织方式。用125I标记的16S rRNA、125I标记的23S rRNA和[32P]tRNA与来自克隆叶绿体DNA片段的限制性核酸酶片段进行杂交。16S和23S rRNA的编码区分别定位在1.75和3.1 kbp范围内。转移rRNA基因位于16S至23S rRNA间隔区。核糖体RNA和tRNA在6.2 kbp串联重复的rRNA基因区域内的同一条DNA链上编码。基因顺序为16S rRNA--tRNAS--23S rRNA--5S rRNA，在叶绿体DNA中重复三次。每组基因聚集在一个5.0 kbp的区域内。如果它们都是单个转录单位的一部分，那么基因的组织方式和大小符合预期。
