纤细裸藻叶绿体DNA的限制性内切酶图谱。

Restriction endonuclease map of Euglena gracilis chloroplast DNA.

作者信息

Gray P W, Hallick R B

出版信息

Biochemistry. 1977 Apr 19;16(8):1665-71. doi: 10.1021/bi00627a022.

Abstract

A physical map of the Euglena gracilis chloroplast genome has been constructed, based on cleavage sites of Euglena gracilis chloroplast DNA treated with bacterial restriction endonucleases. Covalently close, circular chloroplast DNA is cleaved by restriction endonuclease SalI into three fragments and by restriction endonuclease BamHI into six fragments. These nine cleavage sites have been ordered by fragment molecular weight analysis, double digestions, partial digestions, and by digestion studies of isolated DNA fragments. A fragment pattern of the products of EcoRI restriction endonuclease digestion of Euglena chloroplast DNA is also described. One of these fragments has been located on the cleavage site map.

摘要

基于用细菌限制性内切酶处理的纤细裸藻叶绿体DNA的切割位点，构建了纤细裸藻叶绿体基因组的物理图谱。共价闭合的环状叶绿体DNA被限制性内切酶SalI切割成三个片段，被限制性内切酶BamHI切割成六个片段。通过片段分子量分析、双酶切、部分酶切以及对分离的DNA片段的酶切研究，已确定了这九个切割位点的顺序。还描述了纤细裸藻叶绿体DNA经EcoRI限制性内切酶消化产物的片段模式。其中一个片段已定位在切割位点图谱上。