Stiegler G L, Matthews H M, Bingham S E, Hallick R B
Nucleic Acids Res. 1982 Jun 11;10(11):3427-44. doi: 10.1093/nar/10.11.3427.
The gene for the large subunit (LS) of ribulose-1,5,-bisphosphate carboxylase of Euglena gracilis Z chloroplast DNA has been mapped by heterologous hybridization with DNA restriction fragments containing internal sequences from the Zea mays and Chlamydomonas reinhardii LS genes. The Euglena LS gene which has the same polarity as the Euglena rRNA genes has been located with respect to Pst I, Pvu I, and HindIII sites within the Eco RI fragment Eco A. The region of Euglena chloroplast DNA complementary to an 887 bp internal fragment from the Chlamydomonas chloroplast LS gene is interrupted by a 0.5-1.1 kbp non-complementary sequence. This is the first chloroplast protein gene located on the Euglena genome, and the first evidence for an intervening sequence within any chloroplast protein gene.
通过与含有玉米和莱茵衣藻1,5 - 二磷酸核酮糖羧化酶大亚基（LS）基因内部序列的DNA限制片段进行异源杂交，对纤细裸藻Z叶绿体DNA中该酶大亚基基因进行了定位。与纤细裸藻rRNA基因极性相同的纤细裸藻LS基因，已相对于Eco RI片段Eco A内的Pst I、Pvu I和HindIII位点进行了定位。纤细裸藻叶绿体DNA中与衣藻叶绿体LS基因一个887 bp内部片段互补的区域，被一个0.5 - 1.1 kbp的非互补序列中断。这是定位在纤细裸藻基因组上的首个叶绿体蛋白基因，也是任何叶绿体蛋白基因中存在间隔序列的首个证据。
