• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过 2'-磷酸单酯、3'，5'-磷酸二酯键将内源性 tRNA 3' 半分子连接到其相应的 5' 半分子，在衣藻提取物中。

Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.

机构信息

Institut für Biochemie, Universität Würzburg, Röntgenring 11, D-8700 Würzburg, FRG.

出版信息

EMBO J. 1983;2(4):605-10. doi: 10.1002/j.1460-2075.1983.tb01470.x.

DOI:10.1002/j.1460-2075.1983.tb01470.x
PMID:16453445
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC555068/
Abstract

tRNA preparations from Chlamydomonas and wheat germ contain small amounts of tRNA 5' halves and corresponding 3' halves. Incubation of cell-free extracts from the two sources with [gamma-P]ATP yielded 5'-P-labeled tRNA 3' halves which were joined to their corresponding 5' counterparts to form mature tRNA containing 2'-phosphomonoester,3', 5'-phosphodiester bonds. tRNA 3' halves labelled with T4 kinase were purified, sequenced and also joined to their 5' counterparts. It is proposed that these tRNA halves may be intermediates of the tRNA splicing process, and that the RNA kinase and ligase activities observed here are part of the tRNA splicing complex.

摘要

来自衣藻和麦胚的 tRNA 制剂含有少量的 tRNA 5' 片段和相应的 3' 片段。用 [γ-P]ATP 孵育这两种来源的无细胞提取物，得到了 5'-P 标记的 tRNA 3' 片段，这些片段与相应的 5' 片段连接，形成含有 2'-磷酸单酯、3'、5'-磷酸二酯键的成熟 tRNA。用 T4 激酶标记的 tRNA 3' 片段被纯化、测序，并与它们的 5' 片段连接。据推测，这些 tRNA 片段可能是 tRNA 剪接过程的中间产物，并且这里观察到的 RNA 激酶和连接酶活性是 tRNA 剪接复合物的一部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/39128ac4c1bc/emboj00257-0123-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/694db8336037/emboj00257-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/cde2edf0b770/emboj00257-0121-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/0a6b2715bd27/emboj00257-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/5e511ac180d6/emboj00257-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/0c9463fb2fcb/emboj00257-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/39128ac4c1bc/emboj00257-0123-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/694db8336037/emboj00257-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/cde2edf0b770/emboj00257-0121-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/0a6b2715bd27/emboj00257-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/5e511ac180d6/emboj00257-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/0c9463fb2fcb/emboj00257-0123-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2677/555068/39128ac4c1bc/emboj00257-0123-c.jpg

相似文献

1
Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.通过 2'-磷酸单酯、3'，5'-磷酸二酯键将内源性 tRNA 3' 半分子连接到其相应的 5' 半分子，在衣藻提取物中。
EMBO J. 1983;2(4):605-10. doi: 10.1002/j.1460-2075.1983.tb01470.x.
2
An RNA ligase from wheat germ which participates in transfer RNA splicing in vitro.一种来自小麦胚芽的RNA连接酶，其在体外参与转运RNA剪接。
J Biol Chem. 1983 Jul 10;258(13):8365-73.
3
Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.通过来自小麦胚芽和衣藻的一种新型RNA连接酶，经由2'-磷酸单酯、3',5'-磷酸二酯键实现线性类病毒RNA的环化。
Nucleic Acids Res. 1982 Dec 11;10(23):7521-9. doi: 10.1093/nar/10.23.7521.
4
Assembly of a tRNA splicing complex: evidence for concerted excision and joining steps in splicing in vitro.tRNA剪接复合体的组装：体外剪接中协同切除和连接步骤的证据。
Mol Cell Biol. 1986 Feb;6(2):635-44. doi: 10.1128/mcb.6.2.635-644.1986.
5
Archaeal 3'-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation.古菌 3'-磷酸 RNA 剪接连接酶的特性鉴定了 tRNA 成熟过程中的缺失组分。
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1290-5. doi: 10.1073/pnas.1018307108. Epub 2011 Jan 5.
6
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.通过2'-磷酸单酯、3',5'-磷酸二酯键进行RNA连接：2',3'-环磷酸末端的要求以及5'-羟基多核苷酸激酶的参与
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8. doi: 10.1073/pnas.79.5.1474.
7
RNA ligase reaction products in plasmolyzed Escherichia coli cells infected by T4 bacteriophage.被T4噬菌体感染的质壁分离大肠杆菌细胞中的RNA连接酶反应产物。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5430-4. doi: 10.1073/pnas.76.11.5430.
8
A cell-free plant extract for accurate pre-tRNA processing, splicing and modification.一种用于精确进行前体tRNA加工、剪接和修饰的无细胞植物提取物。
EMBO J. 1987 Sep;6(9):2811-8. doi: 10.1002/j.1460-2075.1987.tb02577.x.
9
Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins.植物tRNA连接酶是多功能酶，其在序列和底物特异性上与其他系统发育起源的RNA连接酶有所不同。
Nucleic Acids Res. 2005 Jan 14;33(1):388-99. doi: 10.1093/nar/gki174. Print 2005.
10
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.小麦胚芽中一种新型RNA连接酶形成2'-磷酸单酯、3',5'-磷酸二酯键。
Nature. 1981;293(5828):112-6. doi: 10.1038/293112a0.

引用本文的文献

1
Traversing the RNA world.穿越RNA世界。
J Biol Chem. 2017 May 19;292(20):8122-8135. doi: 10.1074/jbc.X117.789065. Epub 2017 Apr 5.
2
On the evolution and expression of Chlamydomonas reinhardtii nucleus-encoded transfer RNA genes.莱茵衣藻细胞核编码的转运RNA基因的进化与表达
Genetics. 2008 May;179(1):113-23. doi: 10.1534/genetics.107.085688.
3
The molecular basis for the differential translation of TMV RNA in tobacco protoplasts and wheat germ extracts.TMV RNA 在烟草原生质体和小麦胚芽提取物中差异翻译的分子基础。

本文引用的文献

1
Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ.小麦胚 RNA 连接酶对类病毒前体 RNA 的无细胞环化作用。
Science. 1982 Sep 17;217(4565):1147-9. doi: 10.1126/science.217.4565.1147.
2
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
3
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
EMBO J. 1984 May;3(5):1091-6. doi: 10.1002/j.1460-2075.1984.tb01934.x.
4
UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs with suppressor activity from tobacco plants.TMV RNA 翻译中的 UAG 通读：从烟草植物中分离和测序两种具有抑制活性的 tRNA。
EMBO J. 1984 Feb;3(2):351-6. doi: 10.1002/j.1460-2075.1984.tb01810.x.
5
Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins.植物tRNA连接酶是多功能酶，其在序列和底物特异性上与其他系统发育起源的RNA连接酶有所不同。
Nucleic Acids Res. 2005 Jan 14;33(1):388-99. doi: 10.1093/nar/gki174. Print 2005.
6
Junction phosphate is derived from the precursor in the tRNA spliced by the archaeon Haloferax volcanii cell extract.连接磷酸是由嗜盐嗜碱古菌沃氏嗜盐菌细胞提取物剪接的tRNA前体衍生而来。
RNA. 2000 Jul;6(7):1019-30. doi: 10.1017/s1355838200000613.
7
A cell-free plant extract for accurate pre-tRNA processing, splicing and modification.一种用于精确进行前体tRNA加工、剪接和修饰的无细胞植物提取物。
EMBO J. 1987 Sep;6(9):2811-8. doi: 10.1002/j.1460-2075.1987.tb02577.x.
8
A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways.一个人类和一个含有内含子的植物tRNATyr基因在HeLa细胞提取物中均被转录，但沿着不同的途径进行剪接。
EMBO J. 1987 Jan;6(1):35-41. doi: 10.1002/j.1460-2075.1987.tb04715.x.
9
Common structural features of the genes for two stable RNAs from Halobacterium halobium.嗜盐栖热菌两种稳定RNA基因的共同结构特征。
Nucleic Acids Res. 1985 Jan 11;13(1):31-43. doi: 10.1093/nar/13.1.31.
10
Cyclization of RNA 3'-terminal phosphate by cyclase from HeLa cells proceeds via formation of N(3')pp(5')A activated intermediate.来自HeLa细胞的环化酶使RNA 3'-末端磷酸环化是通过形成N(3')pp(5')A活化中间体进行的。
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1316-20. doi: 10.1073/pnas.82.5.1316.
小麦胚芽中一种新型RNA连接酶形成2'-磷酸单酯、3',5'-磷酸二酯键。
Nature. 1981;293(5828):112-6. doi: 10.1038/293112a0.
4
Speculations on RNA splicing.关于RNA剪接的推测。
Cell. 1981 Mar;23(3):643-6. doi: 10.1016/0092-8674(81)90425-6.
5
Intranuclear location of the tRNA splicing enzymes.tRNA剪接酶的核内定位。
Cell. 1981 Jan;23(1):89-93. doi: 10.1016/0092-8674(81)90273-7.
6
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.显微注射非洲爪蟾卵母细胞中的RNA加工。剪接转运RNA中碱基修饰的顺序添加。
J Mol Biol. 1981 Jan 15;145(2):405-20. doi: 10.1016/0022-2836(81)90212-6.
7
Nuclear ligation of RNA 5'-OH kinase products in tRNA.转运RNA中RNA 5'-羟基激酶产物的核连接
Mol Cell Biol. 1982 Mar;2(3):241-9. doi: 10.1128/mcb.2.3.241-249.1982.
8
Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid.柑橘裂皮病和菊花矮化类病毒的核苷酸序列及二级结构
Eur J Biochem. 1982 Jan;121(2):249-57. doi: 10.1111/j.1432-1033.1982.tb05779.x.
9
Rapid cataloging of ribonuclease T1 resistant oligonucleotides from ribosomal RNAs for phylogenetic studies.用于系统发育研究的核糖体RNA中核糖核酸酶T1抗性寡核苷酸的快速编目。
J Mol Evol. 1981;17(4):227-36. doi: 10.1007/BF01732760.
10
Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors.核糖核酸酶“XlaI”，一种来自非洲爪蟾卵母细胞的活性物质，可从酵母转移核糖核酸前体中切除间隔序列。
Mol Cell Biol. 1981 Mar;1(3):269-80. doi: 10.1128/mcb.1.3.269-280.1981.