Science. 1982 Sep 17;217(4565):1147-9. doi: 10.1126/science.217.4565.1147.
Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ. Linear viroid molecules are efficiently converted to circular molecules (circles) which are indistinguishable by electrophoretic mobility and two-dimensional oligonucleotide pattern from viroid circles extracted from infected plants. In light of recent evidence for multimeric viroid replication intermediates, cleavage followed by RNA ligation by a cellular enzyme may (i) be a normal step in the viroid life cycle and (ii) may also reflect cellular events.
线性、马铃薯纺锤块茎类病毒 RNA 已被用作从小麦胚芽中纯化的 RNA 连接酶的底物。线性类病毒分子有效地转化为环形分子（环），其在电泳迁移率和二维寡核苷酸图谱上与从感染植物中提取的类病毒环无法区分。鉴于最近关于多聚类病毒复制中间体的证据，细胞酶的切割随后的 RNA 连接可能是类病毒生命周期中的一个正常步骤，并且还可能反映细胞事件。
