Dildrop R, Brüggemann M, Radbruch A, Rajewsky K, Beyreuther K
EMBO J. 1982;1(5):635-40. doi: 10.1002/j.1460-2075.1982.tb01220.x.
The mouse hybridoma line B1-8.delta 1 secretes a monoclonal IgD, lambda 1 anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibody with defined idiotypic determinants. Two spontaneous V-region variants (B1-8.V1/V2) with altered idiotope pattern were selected and the structural variation was located to the variable region of the heavy chain. The amino acid sequences of the B1-8. delta 1 and variant heavy chain V regions were determined. The variant VH regions are identical. Wild-type and variant VH regions differ in 10 positions. Single amino acid exchanges are found in the first and second framework at positions 20 and 43. The majority of replacements (eight substitutions) is clustered in the second complementary-determining region (CDR 2). There are no differences in CDR 1 and CRD 3 and the JH region. The variant, which at first glance appears to have undergone a series of point mutations, arose by recombination, possibly gene conversion, between the rearranged VDJ gene of the wild-type (B1-8.delta 1) and a neighbouring germ line VH gene encoding all of the substitutions.
小鼠杂交瘤细胞系B1-8.δ1分泌一种具有明确独特型决定簇的单克隆IgD、λ1抗（4-羟基-3-硝基苯基）乙酰（NP）抗体。筛选出了两种独特型模式改变的自发V区变体（B1-8.V1/V2），结构变异定位于重链可变区。测定了B1-8.δ1和变体重链V区的氨基酸序列。变体VH区是相同的。野生型和变体VH区在10个位置存在差异。在第一和第二骨架区的第20和43位发现单个氨基酸交换。大多数替换（8个替换）集中在第二互补决定区（CDR 2）。CDR 1、CRD 3和JH区没有差异。乍一看似乎经历了一系列点突变的变体，是通过野生型（B1-8.δ1）重排的VDJ基因与编码所有替换的相邻种系VH基因之间的重组（可能是基因转换）产生的。
