Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7.
EMBO J. 1984 Apr;3(4):745-9. doi: 10.1002/j.1460-2075.1984.tb01878.x.
The response to heat shock was examined in seven archaebacterial strains from the genus Halobacterium. Upon heat shock each strain preferentially synthesized a limited number of proteins which fell into three narrow mol. wt. ranges. Further examination of the heat-shock response in H. volcanii revealed that heat-shock protein (hsp) synthesis was greatest at 60 degrees C. Synthesis of hsps at this induction temperature was both rapid and transient. Cells recovered their normal protein synthesis patterns rapidly upon returning to their normal growth temperature following heat shock. H. volcanii cells also responded with a ;heat shock-like' response to salt dilution, a natural environmental stress for these organisms. These results indicate that the heat shock or stress response which is charactertistic of eukaryotic and eubacterial cells is also present among members of the archaebacterial genus Halobacterium.
七种来自嗜盐古菌属的古细菌菌株的热休克反应被检测。热休克后，每种菌株优先合成有限数量的蛋白质，这些蛋白质分为三个狭窄的分子量范围。对嗜热古菌 H. volcanii 的热休克反应的进一步研究表明，热休克蛋白（hsp）的合成在 60°C 时最大。在这种诱导温度下，hsps 的合成既迅速又短暂。在经历热休克后回到正常生长温度时，细胞迅速恢复正常的蛋白质合成模式。嗜热古菌 H. volcanii 细胞也对盐稀释产生“热休克样”反应，这是这些生物的自然环境应激。这些结果表明，真核生物和原核生物细胞特有的热休克或应激反应也存在于嗜盐古菌属的成员中。
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