Voellmy R, Bromley P, Kocher H P
J Biol Chem. 1983 Mar 25;258(6):3516-22.
Effects of heat treatments on chick embryo fibroblasts, Drosophila embryonic cells, and human lymphoblastoid cells have been compared. Cells from all three species synthesize large heat-shock proteins (hsps) with Mr = 70,000 and 84,000-85,000. Different small hsps with Mr between 22,000 and 27,000 are made at high rates in heat-treated chicken and Drosophila cells but could not be observed in human cells. The structural features of the large hsps from cells of the different organisms were compared by three methods of peptide mapping, namely the examination of tryptic digests by two-dimensional thin layer chromatography or by high pressure liquid chromatography and of incomplete V8 digests by polyacrylamide gel electrophoresis. The Mr = 84,000-85,000 polypeptides from all three organisms are closely related, the chicken and human polypeptides having many peptides in common. The relationship between the Mr = 70,000 polypeptides of the different organisms appears to be less close; possible explanations for this latter result are discussed. Rates of synthesis of total as well as poly(A)+ RNA are much lower in heat-treated than in untreated cells of all three organisms. Heat treatments induce dramatic changes in the shape of chick embryo fibroblasts as seen by microscopic examination. Human lymphoblastoid cells do not show changes in shape.
已对热处理对鸡胚成纤维细胞、果蝇胚胎细胞和人淋巴母细胞的影响进行了比较。来自这三种物种的细胞均能合成分子量为70,000以及84,000 - 85,000的大型热休克蛋白（hsps）。分子量在22,000至27,000之间的不同小型hsps在热处理的鸡和果蝇细胞中大量合成，但在人细胞中未观察到。通过三种肽图谱分析方法对不同生物体细胞中的大型hsps的结构特征进行了比较，这三种方法分别是通过二维薄层色谱法或高压液相色谱法检查胰蛋白酶消化产物，以及通过聚丙烯酰胺凝胶电泳检查不完全V8消化产物。来自所有三种生物体的分子量为84,000 - 85,000的多肽密切相关，鸡和人的多肽有许多共同的肽段。不同生物体中分子量为70,000的多肽之间的关系似乎没那么紧密；文中讨论了这一结果的可能解释。在所有三种生物体中，热处理细胞中总的以及聚腺苷酸化（poly(A)+）RNA的合成速率均远低于未处理细胞。通过显微镜检查可以看到，热处理会使鸡胚成纤维细胞的形状发生显著变化。人淋巴母细胞的形状未显示出变化。
