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金鱼草的 17-kb Tam1 元件在整合到查尔酮合酶基因时会引起 3-bp 的重复。

The 17-kb Tam1 element of Antirrhinum majus induces a 3-bp duplication upon integration into the chalcone synthase gene.

机构信息

Max-Planck-Institut für Züchtungsforschung, Egelspfad, 5000 Köln 30, FRG.

出版信息

EMBO J. 1984 May;3(5):1015-9. doi: 10.1002/j.1460-2075.1984.tb01921.x.

DOI:10.1002/j.1460-2075.1984.tb01921.x
PMID:16453519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557465/
Abstract

The DNA sequence of the termini and the flanking regions of the 17-kb transposable element Tam1 was determined. Tam1 is integrated in the chalcone synthase gene of the niv-53 mutant of Antirrhinum majus. The element has a 13-bp perfect inverted repeat at its termini and appears to induce a 3-bp duplication of the target site upon integration. The DNA sequence of a niv revertant was analyzed and found to differ from the wild-type sequence by an additional 2 bp that seem to derive from the target site duplication. Stretches of homologous sequences have been found between the ends of Tam1, within each terminus of the element, and between the termini and target site sequences. Structural similarities between the ends of Tam1 and the Spm-18 element of Zea mays reflect a possible horizontal spread of a common progenitor.

摘要

确定了 17kb 可移动元件 Tam1 的末端和侧翼区域的 DNA 序列。Tam1 整合在 Antirrhinum majus 的 niv-53 突变体的查尔酮合酶基因中。该元件在其末端具有 13 个碱基对的完美反向重复，并且似乎在整合时诱导靶位点的 3 个碱基对重复。分析了 niv 回复突变体的 DNA 序列，发现与野生型序列有另外的 2 个碱基对不同，这些碱基似乎来自靶位点重复。在 Tam1 的末端、元件的每个末端以及末端和靶位点序列之间发现了 Tam1 之间的同源序列。Tam1 的末端与玉米 Spm-18 元件之间的结构相似性反映了一个共同祖先的可能水平传播。

相似文献

1
The 17-kb Tam1 element of Antirrhinum majus induces a 3-bp duplication upon integration into the chalcone synthase gene.金鱼草的 17-kb Tam1 元件在整合到查尔酮合酶基因时会引起 3-bp 的重复。
EMBO J. 1984 May;3(5):1015-9. doi: 10.1002/j.1460-2075.1984.tb01921.x.
2
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本文引用的文献

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