Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829 Köln, Germany.
BMC Plant Biol. 2010 Dec 15;10:275. doi: 10.1186/1471-2229-10-275.
Genetic recombination maps provide important frameworks for comparative genomics, identifying gene functions, assembling genome sequences and for breeding. The molecular recombination map currently available for the model eudicot Antirrhinum majus is the result of a cross with Antirrhinum molle, limiting its usefulness within A. majus.
We created a molecular linkage map of A. majus based on segregation of markers in the F2 population of two inbred lab strains of A. majus. The resulting map consisted of over 300 markers in eight linkage groups, which could be aligned with a classical recombination map and the A. majus karyotype. The distribution of recombination frequencies and distorted transmission of parental alleles differed from those of a previous inter-species hybrid. The differences varied in magnitude and direction between chromosomes, suggesting that they had multiple causes. The map, which covered an estimated of 95% of the genome with an average interval of 2 cM, was used to analyze the distribution of a newly discovered family of MITE transposons and tested for its utility in positioning seven mutations that affect aspects of plant size.
The current map has an estimated interval of 1.28 Mb between markers. It shows a lower level of transmission ratio distortion and a longer length than the previous inter-species map, making it potentially more useful. The molecular recombination map further indicates that the IDLE MITE transposons are distributed throughout the genome and are relatively stable. The map proved effective in mapping classical morphological mutations of A. majus.
遗传重组图谱为比较基因组学、鉴定基因功能、组装基因组序列和进行育种提供了重要框架。模型真双子叶植物金鱼草的分子重组图谱是与金鱼草杂交的结果，限制了其在金鱼草中的应用。
我们基于两个金鱼草近交系 F2 群体中标记的分离，创建了金鱼草的分子连锁图谱。该图谱由 8 个连锁群中的 300 多个标记组成，可与经典的重组图谱和金鱼草核型相匹配。重组频率的分布和亲本等位基因的扭曲传递与之前的种间杂种不同。染色体之间的差异在幅度和方向上有所不同，表明它们有多种原因。该图谱覆盖了估计基因组的 95%，平均间隔为 2cM，用于分析新发现的一类 MITE 转座子的分布，并测试其定位影响植物大小的七个突变的能力。
当前的图谱标记之间的估计间隔为 1.28Mb。它显示出比以前的种间图谱更低的传递率失真和更长的长度，因此更具潜力。分子重组图谱进一步表明，IDLE MITE 转座子分布在整个基因组中，并且相对稳定。该图谱在定位金鱼草经典形态突变方面非常有效。
