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1
Sequence analysis of the insertion element ISH1.8 and of associated structural changes in the genome of phage PhiH of the archaebacterium Halobacterium halobium.插入元件 ISH1.8 的序列分析及其在古细菌盐杆菌噬菌体 PhiH 基因组中相关结构变化的研究。
EMBO J. 1984 Aug;3(8):1717-22. doi: 10.1002/j.1460-2075.1984.tb02037.x.
2
An immune strain of Halobacterium halobium carries the invertible L segment of phage PhiH as a plasmid.一种免疫的盐杆菌（Halobacterium halobium）菌株携带可反转的噬菌体 PhiH 的 L 片段作为质粒。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1017-20. doi: 10.1073/pnas.81.4.1017.
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A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene.来自嗜盐嗜盐菌的一种转座元件，它会使细菌视紫红质基因失活。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7268-72. doi: 10.1073/pnas.79.23.7268.
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High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements.嗜盐菌视紫红质基因中的高频自发突变由转座元件介导。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2201-5. doi: 10.1073/pnas.80.8.2201.
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ISH51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, Halobacterium volcanii.ISH51：嗜盐古菌火山栖嗜盐菌基因组中一个类似插入序列元件的大型退化家族。
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IS1630 of Mycoplasma fermentans, a novel IS30-type insertion element that targets and duplicates inverted repeats of variable length and sequence during insertion.发酵支原体的IS1630，一种新型的IS30型插入元件，在插入过程中靶向并复制长度和序列可变的反向重复序列。
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Distribution and characterization of plasmid-related sequences in the chromosomal DNA of different thermophilic Methanobacterium strains.
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Methanogens and the diversity of archaebacteria.产甲烷菌与古细菌的多样性
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Insertion of an intermediate repetitive sequence into a sea urchin histone-gene spacer.一个中间重复序列插入海胆组蛋白基因间隔区。
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ISH51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, Halobacterium volcanii.ISH51：嗜盐古菌火山栖嗜盐菌基因组中一个类似插入序列元件的大型退化家族。
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Restoration of bacterioopsin gene expression in a revertant of Halobacterium halobium.嗜盐栖热菌一个回复突变体中细菌视紫红质基因表达的恢复
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8
Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545.接合性穿梭转座子Tn1545末端的核苷酸序列。
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Transposition burst of the ISH27 insertion element family in Halobacterium halobium.嗜盐栖热菌中ISH27插入元件家族的转座爆发。
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10
Nucleotide sequence of ISH11, a new Halobacterium halobium insertion element isolated from the plasmid pGRB1.ISH11的核苷酸序列，ISH11是一种从质粒pGRB1中分离出的新型嗜盐栖热菌插入元件。
Nucleic Acids Res. 1990 Nov 25;18(22):6699. doi: 10.1093/nar/18.22.6699.

本文引用的文献

1
An immune strain of Halobacterium halobium carries the invertible L segment of phage PhiH as a plasmid.一种免疫的盐杆菌（Halobacterium halobium）菌株携带可反转的噬菌体 PhiH 的 L 片段作为质粒。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1017-20. doi: 10.1073/pnas.81.4.1017.
2
Halobacterium halobium phage øH.嗜盐菌噬菌体 øH.
EMBO J. 1982;1(1):87-92. doi: 10.1002/j.1460-2075.1982.tb01129.x.
3
A new computer method for the storage and manipulation of DNA gel reading data.一种用于存储和处理DNA凝胶读数数据的新型计算机方法。
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
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Bacteriophages of Halobacterium halobium: isolated from fermented fish sauce and primary characterization.
Can J Microbiol. 1982 Aug;28(8):916-21. doi: 10.1139/m82-138.
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High-frequency genomic rearrangements involving archaebacterial repeat sequence elements.涉及古细菌重复序列元件的高频基因组重排。
Nature. 1982 Sep 9;299(5879):182-5. doi: 10.1038/299182a0.
6
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
7
Characterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium.影响嗜盐菌中细菌视紫红质基因表达的插入序列的特征分析。
Nucleic Acids Res. 1984 Mar 12;12(5):2489-97. doi: 10.1093/nar/12.5.2489.
8
Transposition of Tn554 does not generate a target duplication.Tn554的转座不会产生靶序列重复。
Nature. 1984;307(5948):292-4. doi: 10.1038/307292a0.
9
Structure of the archaebacterial transposable element ISH50.古细菌转座因子ISH50的结构
Nucleic Acids Res. 1983 Jun 25;11(12):4195-9. doi: 10.1093/nar/11.12.4195.
10
Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.经超声处理的DNA的随机亚克隆：在鸟枪法DNA序列分析中的应用
Anal Biochem. 1983 Feb 15;129(1):216-23. doi: 10.1016/0003-2697(83)90072-6.

插入元件 ISH1.8 的序列分析及其在古细菌盐杆菌噬菌体 PhiH 基因组中相关结构变化的研究。

Sequence analysis of the insertion element ISH1.8 and of associated structural changes in the genome of phage PhiH of the archaebacterium Halobacterium halobium.

机构信息

Max-Planck-Institut für Biochemie, 8033 Martinsried, FRG.

出版信息

EMBO J. 1984 Aug;3(8):1717-22. doi: 10.1002/j.1460-2075.1984.tb02037.x.

DOI:10.1002/j.1460-2075.1984.tb02037.x
PMID:16453543
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557587/
Abstract

We have sequenced the insertion element ISH1.8 which can be present in one or two copies in the genome of phage PhiH of Halobacterium halobium. ISH1.8 is 1895 bp long, has no inverted repeat at its ends, and one only of the two copies is flanked by two 5-bp duplications. An 8-bp sequence composed of 4 bp from each end of ISH1.8 is present in both sites lacking the element. This 8-bp sequence could either be a specific insertion sequence or a part of the element that is left behind upon deletion. The plasmid pPhiHL, consisting of the invertible L segment of the phage genome which is, in PhiH2 and PhiH5, flanked by two copies of ISH1.8, contains 112 bp of ISH1.8 and is released from the phage genome by recombination within a direct repeat of 9 bp. This 9-bp sequence (TCCCGCCCT) exists as an inverted repeat in ISH1.8 and therefore as two distinct repeats in phage genomes containing two copies of ISH1.8 in inverted orientation.

摘要

我们已经对插入元件 ISH1.8 进行了测序，该元件可在嗜盐菌噬菌体 PhiH 的基因组中存在一个或两个拷贝。ISH1.8 长 1895 个碱基，其两端没有反向重复序列，两个拷贝中只有一个被两个 5 碱基重复序列侧翼。ISH1.8 两端的 8 个碱基序列（每个末端各有 4 个碱基）存在于缺失元件的两个位点中。该 8 个碱基序列可能是一个特定的插入序列，也可能是元件删除后留下的一部分。质粒 pPhiHL 由噬菌体基因组的可翻转 L 片段组成，在 PhiH2 和 PhiH5 中，该片段被两个 ISH1.8 拷贝侧翼包围，包含 112 个碱基的 ISH1.8，通过 9 个碱基的直接重复内的重组从噬菌体基因组中释放出来。该 9 个碱基序列（TCCCGCCCT）在 ISH1.8 中作为反向重复存在，因此在含有两个 ISH1.8 拷贝的噬菌体基因组中以两个不同的重复存在，它们的方向相反。