ISH51：嗜盐古菌火山栖嗜盐菌基因组中一个类似插入序列元件的大型退化家族。

Hofman J D, Schalkwyk L C, Doolittle W F

Nucleic Acids Res. 1986 Sep 11;14(17):6983-7000. doi: 10.1093/nar/14.17.6983.

We describe a new family of repetitive elements in the genome of the archaebacterium Halobacterium volcanii. There are some 20-30 copies of this element, which we designate ISH51. Sequenced copies show typical insertion sequence characteristics (terminal inverted repeats, direct flanking repeats of "target site" DNA). However, members of the ISH51 family are highly heterogeneous, showing on average only 85% primary sequence homology; and some genomic copies appear to be severely truncated. Some ISH51 elements are clustered together in regions of relatively AT-rich DNA. There are at least five such AT-rich "islands" in the H. volcanii genome. Repetitive sequences homologous to ISH51 are found in the genomes of most Halobacterium and Halococcus species.

我们描述了嗜盐古菌沃氏嗜盐菌基因组中一个新的重复元件家族。该元件约有20 - 30个拷贝，我们将其命名为ISH51。测序的拷贝显示出典型的插入序列特征（末端反向重复序列、“靶位点”DNA的直接侧翼重复序列）。然而，ISH51家族成员高度异质，平均仅显示85%的一级序列同源性；并且一些基因组拷贝似乎严重截短。一些ISH51元件在相对富含AT的DNA区域聚集在一起。沃氏嗜盐菌基因组中至少有五个这样富含AT的“岛”。在大多数嗜盐菌和盐球菌物种的基因组中都发现了与ISH51同源的重复序列。