Plant Disease Resistance Research Unit, Agricultural Research Service, United States Department of Agriculture, and Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1978 Sep;62(3):363-7. doi: 10.1104/pp.62.3.363.
Extracts prepared from sunflower (Helianthus annuus L.) crown gall tissues induced by Agrobacterium tumefaciens strains C58 and T37 (nopaline utilizers) catalyze the synthesis of nopaline and ornaline. These compounds are not synthesized in extracts of crown gall tissues induced by strains B6, 15955 (octopine utilizers), and AT1 (utilizes neither octopine nor nopaline) or in extracts of habituated sunflower callus. Both synthetic activities require NADPH, alpha-ketoglutarate, and either arginine or ornithine; histidine and lysine will not substitute. Incorporation of arginine or ornithine into product is inhibited by the other substrate but not by histidine or lysine. On the basis of inhibition and K(m) data, both activities appear to be catalyzed by one enzyme and the same enzyme is apparently present in crown gall tissues induced by strains C58 and T37.
由根癌农杆菌菌株 C58 和 T37（利用胭脂碱）诱导的向日葵冠瘿组织提取物可催化胭脂碱和瓜氨酸的合成。这些化合物不能在由菌株 B6、15955（利用奥克托品）和 AT1（既不利用奥克托品也不利用胭脂碱）诱导的冠瘿组织提取物中合成，也不能在适应的向日葵愈伤组织提取物中合成。这两种合成活性都需要 NADPH、α-酮戊二酸和精氨酸或鸟氨酸；组氨酸和赖氨酸不能替代。精氨酸或鸟氨酸掺入产物被另一种底物抑制，但不被组氨酸或赖氨酸抑制。根据抑制和 K(m)数据，这两种活性似乎都由一种酶催化，并且该酶显然存在于由 C58 和 T37 菌株诱导的冠瘿组织中。
