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果蝇额外性梳基因的克隆及其通过 P 元件介导的基因转移进行鉴定。

Cloning of the extra sex combs gene of Drosophila and its identification by P-element-mediated gene transfer.

机构信息

Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland.

出版信息

EMBO J. 1985 Apr;4(4):979-87. doi: 10.1002/j.1460-2075.1985.tb03727.x.

DOI:10.1002/j.1460-2075.1985.tb03727.x
PMID:16453608
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554288/
Abstract

A large region containing the extra sex combs (esc) gene of Drosophila has been cloned by microdissection from polytene chromosomes and chromosomal walking. Within this DNA, the segment comprising the esc gene has been narrowed down in several steps. First, a deletion of the esc gene, Df(2L)esc, defined a region of 380 kb. Then, a duplication carrying the esc gene, Dp(2;2)GYL, and a deletion not eliminating esc, Df(2L)prd, further reduced this region to 160 kb. Finally, because esc transcripts are expected to be present in follicles but absent in adult males, mapping of follicle and adult male transcripts limited the esc region to 10 kb. Rigorous proof that this DNA contained the esc gene was obtained by P-element-mediated transformation. We conclude that the 12 kb of DNA used for transformation include the esc gene as well as all flanking sequences required for its proper regulation in the female germ line.

摘要

一个包含果蝇额外性梳（esc）基因的大片段区域已通过多线染色体的显微切割和染色体步移技术进行了克隆。在这段 DNA 中，esc 基因的片段已通过若干步骤进行了缩小。首先，esc 基因缺失突变体 Df(2L)esc 界定了一个 380kb 的区域。随后，携带 esc 基因的重复体 Dp(2;2)GYL 和不缺失 esc 基因的缺失体 Df(2L)prd 进一步将此区域缩小到 160kb。最后，由于 esc 转录本预计存在于滤泡中而不存在于成年雄性中，因此滤泡和成年雄性转录本的作图将 esc 区域限制在 10kb 内。通过 P 元素介导的转化获得了该 DNA 包含 esc 基因的严格证据。我们得出结论，用于转化的 12kb DNA 包含 esc 基因以及其在雌性生殖系中正确调控所需的所有侧翼序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/7181e0daccf8/emboj00269-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/3cc6fe8f2153/emboj00269-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/1755f9446075/emboj00269-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/fd523b7685ba/emboj00269-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/e5ebd432195e/emboj00269-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/7181e0daccf8/emboj00269-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/3cc6fe8f2153/emboj00269-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/1755f9446075/emboj00269-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/fd523b7685ba/emboj00269-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/e5ebd432195e/emboj00269-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4d/554288/7181e0daccf8/emboj00269-0135-a.jpg

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