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流式细胞术与细胞培养中病毒分离用于鉴定持续感染牛病毒性腹泻病毒的牛的比较。

Comparison of flow cytometry and virus isolation in cell culture for identification of cattle persistently infected with bovine viral diarrhea virus.

作者信息

Qvist P, Houe H, Aasted B, Meyling A

机构信息

Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.

出版信息

J Clin Microbiol. 1991 Mar;29(3):660-1. doi: 10.1128/jcm.29.3.660-661.1991.

DOI:10.1128/jcm.29.3.660-661.1991
PMID:1645374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269842/
Abstract

Detection of bovine viral diarrhea virus in 143 blood samples by virus isolation in cell culture and flow cytometry was performed. The material included 37 samples later shown to originate from persistently infected cattle. Thirty-three samples were positive by virus isolation, and all 37 samples were positive by the flow cytometric assay.

摘要

通过细胞培养病毒分离和流式细胞术对143份血液样本进行牛病毒性腹泻病毒检测。材料包括37份后来证明来自持续感染牛的样本。33份样本通过病毒分离呈阳性,所有37份样本通过流式细胞术检测呈阳性。

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本文引用的文献

1
Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays.猪血清中猪霍乱病毒和牛病毒性腹泻病毒抗体的检测。使用补体结合试验(CF)、粒子凝集试验(PLA)和核酸粒子凝集试验(NPLA)的比较检测
Acta Vet Scand. 1981;22(1):85-98. doi: 10.1186/BF03547210.
2
Bovine virus diarrhoea-mucosal disease infection in cattle.牛的牛病毒性腹泻-黏膜病感染
Vet Rec. 1985 Sep 7;117(10):240-5. doi: 10.1136/vr.117.10.240.
3
[Production of high titrating antisera against BVD virus from swine and its use for direct immunofluorescence and immunoperoxidase technics].[从猪制备高滴度抗牛病毒性腹泻病毒抗血清及其在直接免疫荧光和免疫过氧化物酶技术中的应用]
Dtsch Tierarztl Wochenschr. 1987 Nov-Dec;94(10):576-80.
4
Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.流式细胞术检测持续感染牛外周血白细胞中的牛病毒性腹泻病毒
Can J Vet Res. 1990 Oct;54(4):469-72.