Max-Planck-Institut für Entwicklungsbiologie, Spemannstr, 35, D-7400 Tübingen, FRG.
EMBO J. 1987 Sep;6(9):2793-801. doi: 10.1002/j.1460-2075.1987.tb02575.x.
Genetic analysis of the Drosophila chromosome region 60 E9-F1 identified two functions affecting embryonic development; gooseberry (gsb), a segment polarity gene, and zipper (zip), an unclassified gene which affects cuticle formation severely. By contrast, molecular analysis revealed five genes with different temporal and spatial patterns of expression in the embryo. Candidate genes for gsb and zip functions were identified. Two adjacent genes are eventually expressed in regular stripes within the posterior region of each segment. One of them is expressed initially in a pair-rule mode; the second gene expresses reduced levels of transcripts in a mutant which leaves the transcribed region and the sequences up to the second gene intact. This observation, the patterns of transcripts in the embryo and the genetic data suggest that both genes are involved in gooseberry segmentation function. zip is expressed in neural tissue and not in epidermal anlagen. Embryos lacking zip activity also develop abnormal neural tissue consistent with the argument that the zip cuticle phenotype is a secondary effect. Additional newly identified genes are expressed in specific domains of the embryo, covering mesoderm anlagen and the dorsal region of embryos at blastoderm stage, respectively.
对果蝇染色体区域 60E9-F1 的遗传分析鉴定出两个影响胚胎发育的功能；gooseberry（gsb），一个节段极性基因，和 zipper（zip），一个严重影响表皮形成的未分类基因。相比之下，分子分析显示在胚胎中有五个具有不同时空表达模式的基因。gsb 和 zip 功能的候选基因被鉴定出来。两个相邻的基因最终在每个节段的后区域以规则的条纹表达。其中一个最初以一对规则模式表达；第二个基因在一个转录区和第二个基因的序列保持完整的突变体中表达的转录本水平降低。这一观察结果，以及胚胎中的转录本模式和遗传数据表明，这两个基因都参与了 gooseberry 的分段功能。zip 在神经组织中表达，而不在表皮原基中表达。缺乏 zip 活性的胚胎也发育出异常的神经组织，这与 zip 表皮表型是一种次级效应的观点一致。另外新鉴定的基因分别在胚胎的特定区域表达，分别覆盖中胚层原基和胚盘期胚胎的背部区域。
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