微管结合蛋白CLIP-170、EB1和p150Glued形成不同的正端复合物。
Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes.
作者信息
Ligon Lee A, Shelly Spencer S, Tokito Mariko K, Holzbaur Erika L F
机构信息
Department of Physiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
FEBS Lett. 2006 Feb 20;580(5):1327-32. doi: 10.1016/j.febslet.2006.01.050. Epub 2006 Jan 26.
Abstract
Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP-170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued, which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo.
摘要
微管正端蛋白CLIP-170和EB1在体内动态追踪生长中的微管尖端。在此，我们在体外研究这些蛋白与微管的关联。CLIP-170结合微管蛋白二聚体并共同组装到生长中的微管中。EB1与微管蛋白二聚体的结合较弱，因此未观察到共同组装。然而，EB1与CLIP-170结合，并与CLIP-170和微管蛋白形成一个共同复合物，该复合物被招募到生长中的微管正端。CLIP-170与EB1之间的相互作用受到相关的CAP-Gly蛋白p150Glued的竞争性抑制，p150Glued在体内也定位于微管正端。基于这些观察结果，我们提出了一个模型，其中不同正端复合物的形成可能在体内对微管动力学产生不同影响。
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本文引用的文献
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