Gilks W R, Oldfield L, Wild P
Medical Research Council Biostatistics Unit, Cambridge, UK.
Br J Cancer Suppl. 1991 Jun;14:3-9.
Methods of data collection for the 2nd Small Cell Lung Cancer Workshop are described, and data reliability is reviewed. The method of cluster analysis of the workshop antibodies is described and discussed. Of the 27,111 results submitted 20,705 were judged to be reliable for analysis and 13,802 of these came from immunohistology experiments. Data derived from immunocytochemistry experiments were somewhat less reproducible than flow cytometry, immunohistology and ELISA experiments. The cluster analysis was developed from methods employed in the leucocyte antigens workshops. Several checks on the methods of cluster analysis and the transformation of data did not substantially alter the final groupings. The workshop confirms that, although there are some methodological difficulties, the cluster analysis can successfully be applied to data derived largely from immunohistology, and thus has applicability to other tumour types.
描述了第二届小细胞肺癌研讨会的数据收集方法，并对数据可靠性进行了审查。描述并讨论了研讨会抗体的聚类分析方法。在提交的27111个结果中，有20705个被判定可用于分析，其中13802个来自免疫组织学实验。免疫细胞化学实验获得的数据的可重复性略低于流式细胞术、免疫组织学和酶联免疫吸附测定实验。聚类分析是根据白细胞抗原研讨会采用的方法发展而来的。对聚类分析方法和数据转换进行的几次检查并没有实质性地改变最终的分组。研讨会证实，尽管存在一些方法上的困难，但聚类分析可以成功地应用于主要来自免疫组织学的数据，因此也适用于其他肿瘤类型。
