Skov B G, Hirsch F R, Hay F G, Olsen J E, Bobrow L G
University Department of Oncology and Pathology, Rigshospitalet, Copenhagen, Denmark.
Br J Cancer Suppl. 1991 Jun;14:46-8.
The presence of 'small cell lung cancer antigens' was evaluated in pretreatment biopsies of primary SCLC, liver metastases, and/or bone marrow metastases from 46 patients. The antigen expression was detected immunohistochemically by applying monoclonal antibodies to routinely formalin-fixed and paraffin embedded tissue. The antibodies applied were second workshop codes: 3(CAM 5.2), 45 (MOV15), 54 (NCCST433), 75 (PE35), 81 (HMFG-1), 95 (LCA1/L38) and HMFG-2 123C3, F4 and MOC-21. High expression was observed for WS 3, 45, 75, 81 and HMFG-2, both in the primaries and metastases. A good correlation was observed between the presence of antigens in primary biopsies and metastases, but there was a general tendency toward a lower proportion of positive tumour cells in the metastases compared to the primaries. For WS 45, 54, 75 and 95 the difference between primaries and bone marrow was statistically significant, and this was also true for WS 45 and 81 in the liver. The clinical relevance is discussed.
对46例患者原发性小细胞肺癌、肝转移瘤和/或骨髓转移瘤的预处理活检样本进行了“小细胞肺癌抗原”检测。通过将单克隆抗体应用于常规福尔马林固定和石蜡包埋组织，免疫组织化学检测抗原表达。所应用的抗体为第二届研讨会编码：3（CAM 5.2）、45（MOV15）、54（NCCST433）、75（PE35）、81（HMFG-1）、95（LCA1/L38）以及HMFG-2 123C3、F4和MOC-21。在原发性肿瘤和转移瘤中均观察到WS 3、45、75、81和HMFG-2的高表达。原发性活检样本和转移瘤中抗原的存在情况之间存在良好的相关性，但与原发性肿瘤相比，转移瘤中阳性肿瘤细胞的比例总体呈下降趋势。对于WS 45、54、75和95，原发性肿瘤与骨髓之间的差异具有统计学意义，对于肝转移中的WS 45和81也是如此。本文讨论了其临床相关性。
