Moss F, Bobrow L, Happerfield L, Souhami R, Beverley P
Chest and Allergy Clinic, St Mary's Hospital, London, UK.
Br J Cancer Suppl. 1991 Jun;14:54-5.
Antigen expression by human small cell lung carcinoma cells in serous effusions was determined by staining smears of cells with a panel of four monoclonal antibodies including UJ13A (cluster 1 antigen) and CAM 5.2 (anti-cytokeratin) using an immunoalkaline phosphatase technique. This was quantified by counting the proportion of stained and unstained tumour cells and was compared with that of small cell lung carcinoma in sections of solid tumour. Differential expression of antigen expression was noted with significantly fewer small cell carcinoma cells in serous effusions staining with UJ13A or CAM 5.2. The reasons for this differential expression are unknown, but may reflect adaptation to a different environment or be a prerequisite for spread to serous space.
采用免疫碱性磷酸酶技术，通过用包括UJ13A（第1簇抗原）和CAM 5.2（抗细胞角蛋白）在内的四种单克隆抗体对细胞涂片进行染色，来测定人小细胞肺癌细胞在浆液性渗出液中的抗原表达。通过计数染色和未染色肿瘤细胞的比例对其进行定量，并与实体瘤切片中的小细胞肺癌进行比较。观察到抗原表达存在差异，浆液性渗出液中用UJ13A或CAM 5.2染色的小细胞癌细胞明显较少。这种差异表达的原因尚不清楚，但可能反映了对不同环境的适应，或者是扩散到浆膜腔的一个先决条件。
