Gao Ya, Jin Tian-bo, Yu Bing, Du Hong, Li Sheng-bin
The State Key Subject for Forensic Sciences, Key Laboratory of National Ministry of Health for Forensic Sciences, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R.China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Feb;23(1):97-9.
To investigate the alleles and genotypes frequency of 10 short tandem repeat (STR) loci on the X chromosome(DXS7423, DXS8378, DXS6799, DXS7424, DXS7130, DXS7132, DXS6789, DXS101, DXS6804, DXS7133) of Tibetan individuals living in Xizang Autonomous Region, Southwest China.
The 10 X-chromosomal STR loci were analyzed with PCR, followed by polyacylamide gel electrophoresis and silver staining.
Among unrelated Tibetan individuals, the numbers of alleles in the 10 X-STR loci(DXS7423, DXS8378, DXS6799, DXS7424, DXS7130, DXS7132, DXS6789, DXS101, DXS6804, DXS7133) were 5, 5, 5, 9, 7, 7, 11, 9, 5 and 4, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium.
The 10 X-chromosomal STRs loci are appropriate for individual identification, for paternity testing involving a female child, and for studies on related disease.
研究中国西南部西藏自治区藏族人群X染色体上10个短串联重复序列(STR)基因座(DXS7423、DXS8378、DXS6799、DXS7424、DXS7130、DXS7132、DXS6789、DXS101、DXS6804、DXS7133)的等位基因及基因型频率。
采用聚合酶链反应(PCR)分析这10个X染色体STR基因座,随后进行聚丙烯酰胺凝胶电泳和银染。
在无关藏族个体中,10个X-STR基因座(DXS7423、DXS8378、DXS6799、DXS7424、DXS7130、DXS7132、DXS6789、DXS101、DXS6804、DXS7133)的等位基因数分别为5、5、5、9、7、7、11、9、5和4。女性的基因型频率符合哈迪-温伯格平衡。
这10个X染色体STR基因座适用于个体识别、涉及女童的亲子鉴定以及相关疾病研究。