[中国汉族人群X染色体上6个短串联重复序列位点的基因多态性]

[Genetic polymorphism of six short tandem repeat loci on the X chromosome in a Han race population in China].

作者信息

Feng Xue, Wang Yan, Fan Ying, Yu Bing, Li Sheng-bin

机构信息

Key Laboratory of National Ministry of Health for Forensic Sciences & Environment and Genes, Diseases Key Laboratory of the Education Ministry, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R.China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Feb;23(1):92-4.

PMID:16456798

Abstract

OBJECTIVE

To investigate the alleles and genotype frequencies of 6 short tandem repeat (STR) loci DXS7130, DXS1214, DXS6799, DXS6804, DXS7424 and DXS7133 on the X chromosome in Chinese Han individuals living in Shaanxi province of Northwest China.

METHODS

The 6 X-chromosomal STR loci were typed by PCR, denatured polyacylamide gel electrophoresis and silver stain.

RESULTS

Among females from Xi'an Han population, the numbers of alleles in the 5 loci (DXS7130, DXS6799, DXS6804, DXS7424 and DXS7133) were detected to be 9, 6, 6, 7, and 7 respectively; the numbers of genotypes were 21, 14, 15, 17 and 12 respectively; and the number of alleles in the DXS1214 was 8. Exact tests demonstrated that genotype frequencies of females had no departure from Hardy-Weinberg equilibrium.

CONCLUSION

The six loci of X-chromosomal STRs are appropriate for individual identification, paternity test involving in female child and for study in related disease.

摘要

目的

研究中国西北陕西省汉族人群X染色体上6个短串联重复序列（STR）基因座DXS7130、DXS1214、DXS6799、DXS6804、DXS7424和DXS7133的等位基因及基因型频率。

方法

采用聚合酶链反应（PCR）、变性聚丙烯酰胺凝胶电泳及银染技术对6个X染色体STR基因座进行分型。

结果

在西安汉族女性人群中，检测到5个基因座（DXS7130、DXS6799、DXS6804、DXS7424和DXS7133）的等位基因数分别为9、6、6、7和7；基因型数分别为21、14、15、17和12；DXS1214的等位基因数为8。确切概率检验表明女性基因型频率符合Hardy-Weinberg平衡。