西安汉族人群X染色体6个STR基因座的遗传多态性分析
[Genetic polymorphism analysis of 6 STR loci on the X chromosome in Xi'an Han population].
作者信息
Yu Bing, Qin Qun-xia, Yan Jin-cheng
机构信息
The National laboratory of the Ministry of Health for Forensic Science, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Fa Yi Xue Za Zhi. 2005 Aug;21(3):188-91.
OBJECTIVE
To investigate the alleles and genotypes frequency of 6 short tandem repeat (STR) loci (DXS8378, DXS7132, DXS6789, DXS101, HPRTB and DXS7423) on the X chromosome in Han population.
METHODS
The six X-chromosome STR loci were analyzed by PCR following polyacylamide gel electrophoresis and silver stain.
RESULTS
Among 120 females from Xi'an Han population, the number of alleles in the 6 loci (DXS8378, DXS7132, DXS6789, DXS101, HPRTB and DXS7423) were 5, 6, 11, 10, 8, and 4 respectively; the number of genotypes in the 6 loci were 10, 17, 29, 32, 22, and 7 respectively; Exact tests demonstrated genotype frequencies in females had no departure from Hardy-Weinberg equilibrium.
CONCLUSION
The six X-chromosome STR loci are appropriate for individual identification, paternity testing involving a female child and for study on related disease.
目的
研究汉族人群X染色体上6个短串联重复序列（STR）基因座（DXS8378、DXS7132、DXS6789、DXS101、HPRTB和DXS7423）的等位基因及基因型频率。
方法
采用聚合酶链反应（PCR）扩增后经聚丙烯酰胺凝胶电泳及银染法对6个X染色体STR基因座进行分析。
结果
在120名西安汉族女性中，6个基因座（DXS8378、DXS7132、DXS6789、DXS101、HPRTB和DXS7423）的等位基因数分别为5、6、11、10、8和4；6个基因座的基因型数分别为10、17、29、32、22和7；确切概率检验表明女性基因型频率符合Hardy-Weinberg平衡。
结论
这6个X染色体STR基因座适用于个体识别、涉及女童的亲子鉴定及相关疾病研究。
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