[中国宁夏地区回族人群X染色体10个STR基因座的遗传多态性调查]

[An investigation for genetic polymorphisms of 10 STR loci on chromosome X of Chinese Hui nationality population in Ningxia region of China].

作者信息

Feng Xue, Xu Ping, Tuo Ya, Yu Bing, Li Sheng-bin

机构信息

Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xioan Jiaotong University, Xioan, Shaanxi, 710061, PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Jun;23(3):346-8.

PMID:16767682

Abstract

OBJECTIVE

To investigate the alleles and genotypes frequency of 10 short tandem repeat (STR) loci (DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378) on X chromosome of Chinese Hui nationality population.

METHODS

The study of 10 STR loci was performed by using the techniques of PCR, polyacylamide gel electrophoresis and silver staining.

RESULTS

Among unrelated Hui individuals, the allele numbers of 10 STR loci DXS101, DXS6789, DXS6799, DXS6804, DXS7130, DXS7132, DXS7133, DXS7423, HPRTB, DXS8378 were 9, 8, 4, 6, 6, 6, 4, 4, 5 or 5 respectively; the numbers of genotypes were 17, 22, 7, 14, 14, 15, 6, 7, 12 or 8 respectively. The distribution of genotypes from these 10 STR systems fitted the Hardy-Weinberg equilibrium (P> 0.05). Polymorphisms information content of 10 STR loci, except for DXS7133 (0.48) and DXS7423 (0.48), ranged from 0.54 (DXS6799) to 0.80 (DXS6789); the power of discrimination were from 0.89 (DXS7133, DXS7423) to 0.99 (DXS6789, DXS7132, DXS101).

CONCLUSION

The loci of 10 STR on chromosome X are appropriate for individual identification, paternity testing involving a female child and for study on related disease.

摘要

目的

研究中国回族人群X染色体上10个短串联重复序列（STR）基因座（DXS101、DXS6789、DXS6799、DXS6804、DXS7130、DXS7132、DXS7133、DXS7423、HPRTB、DXS8378）的等位基因及基因型频率。

方法

采用聚合酶链反应（PCR）、聚丙烯酰胺凝胶电泳及银染技术对10个STR基因座进行研究。

结果

在无关回族个体中，DXS101、DXS6789、DXS6799、DXS6804、DXS7130、DXS7132、DXS7133、DXS7423、HPRTB、DXS8378这10个STR基因座的等位基因数分别为9、8、4、6、6、6、4、4、5或5；基因型数分别为17、22、7、14、14、15、6、7、12或8。这10个STR系统的基因型分布符合哈迪-温伯格平衡（P>0.05）。10个STR基因座中，除DXS7133（0.48）和DXS7423（0.48）外，多态性信息含量范围为0.54（DXS6799）至0.80（DXS6789）；鉴别力为0.89（DXS7133、DXS7423）至0.99（DXS6789、DXS7132、DXS101）。