Kihara Takanori, Hirose Motohiro, Oshima Akira, Ohgushi Hajime
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Biochem Biophys Res Commun. 2006 Mar 24;341(4):1029-35. doi: 10.1016/j.bbrc.2006.01.059. Epub 2006 Jan 25.
We cultured rat mesenchymal stem cells (MSCs) in a medium containing beta-glycerophosphate, ascorbic acid, and dexamethasone to show in vitro osteogenic differentiation of the MSCs. The differentiation was enhanced by adding solubilized type I collagen to the medium as evidenced by higher alkaline phosphatase activity as well as more calcium deposition than that without collagen. The exogenous collagen integrated well with the mineralized bone matrix and maintained the native triple helical structure. These findings indicate that exogenously supplemented type I collagen acts as a component of the extracellular matrix of MSCs, and deposited type I collagen facilitates osteogenic differentiation followed by maturation of mineralized bone matrix.
我们在含有β-甘油磷酸、抗坏血酸和地塞米松的培养基中培养大鼠间充质干细胞（MSC），以展示MSC的体外成骨分化。通过向培养基中添加可溶性I型胶原蛋白，分化得到增强，这表现为碱性磷酸酶活性更高，且钙沉积比不添加胶原蛋白时更多。外源性胶原蛋白与矿化骨基质很好地整合，并保持了天然的三螺旋结构。这些发现表明，外源性补充的I型胶原蛋白作为MSC细胞外基质的一个组成部分，沉积的I型胶原蛋白促进成骨分化，随后矿化骨基质成熟。
